Development of microoptics integrated microfluidic system for cell analysis

• Main Authors: Yu-Jui Fan, 范育睿
• Other Authors: Horn-Jiunn Sheen
• Format: Others
• Language: en_US
• Published: 2014
• Online Access: http://ndltd.ncl.edu.tw/handle/08604427905929214517

博士 === 國立臺灣大學 === 應用力學研究所 === 102 === This thesis reports the microfluidic devices with parallel detection channel embedded microoptics array for versatile high throughput multicolor fluorescence detection. These devices are realized by utilizing solid immersion micro ball lens arrays for high sensitivity and parallel fluorescence detection. For droplet detection, the droplets, produced by a droplet generator in the upstream of device, randomly flow into 64 parallel channels. For cell detection, the cells randomly flow into 32 channel, in which cells got aligned in the center stream. A total throughput of 358,400 cells/s has been accomplished. High refractive index (R.I.) micro ball lenses (R.I.=2.1) are embedded underneath PDMS channels close to cell detection zones in channels. This design permits patterning high N.A. micro ball lenses in a compact fashion for parallel fluorescence detection on a small footprint device. This cell detection device also utilizes 3D microfluidic fabrication to address fluid routing issues in two-dimensional parallel sheath focusing and allows simultaneous pumping of 32 sample channels and 64 sheath flow channels with only two inlets.