A Biosensing Method Based on Nano-Particles’ Brownian Motion Applied to Nanofluidic Preconcentration Chip

• Main Authors: Heng-Chia Chiou, 邱珩嘉
• Other Authors: Horn-Jiunn Sheen
• Format: Others
• Language: zh-TW
• Published: 2014
• Online Access: http://ndltd.ncl.edu.tw/handle/03657647745290000142

碩士 === 國立臺灣大學 === 應用力學研究所 === 102 === In this thesis, we have developed a novel detection method based on capturing preconcentrated plug preconcentrated by preconcentration chip and Brownian motion detection. Applying voltage drop to the preconcentrator which use Nafion as ion-selective membrane can generate ion concentration polarization (ICP) effect. The electroosmosis of second kind which generate by applying the bias voltage, force bulk solution to aggregate the preconcentration plug near the depletion region. Using the Brownian motion which analyze the characteristic of antigen-antibody interactions as detection mechanism to detect the preconcentration plug captured by pneumatic valve. 　　The design and the validation methods and processes of nanofluidic preconcentration chips and pneumatic valve were proposed starting from validation of resistive models, test of ion-selective membrane and test of Brownian motion by micro-Particle Tracking Velocimetry (micro-PTV), to get the onset of detection mechanism. 　　In summary, the presented nanofluidic preconcentrator and pneumatic valve demonstrates biological sample can be preconcentrated in short time and the plug can be captured by valve. With the analysis of loop currents and Brownian motion detection, various low biological sample could be demonstrated in the future.