| Summary: | 碩士 === 國立臺灣科技大學 === 醫學工程研究所 === 102 === In this study, we used the way of casting to mix Silk and PVA together to generate a composite membrane, i.e., Non-porous-Silk Fibroin Protein Membranes (NP-SFPM) and subsequently used NaCl to creat pores in the NP-SFPM (Porous-Silk Fibroin Protein Membranes, P-SFPM),for increasing its permeability. We also added Tea Tree Oil (TTO) to abovementioned membranes and then assessed its efficacy on the treatment of skin acne infection.In this study, we found that silk fibroin protein membranes (SFPM) doesn’t have significant cytotoxicity under appropriate amount of TTO addition. When the content of TTO added to NP-SFPM and P-SFPM up to 25 μL , cell viability decreased to, 67.5 ± 4.0% and 49.3 ± 8.2%. Thus, we choose 3.125 μL, 6.25 μL, and 12.5 μL for the following anti-inflammatory and anti-bacterial studies. The experiments prove that the SFPM exhibits a slight anti-inflammatory effect. In addition, under a small amount of extra TTO addition, our membranes show remarkable ability on inhibiting the NO formation in LPS-induced giant macrophages (Raw 264.7). In inhibiting the proliferation of Propionibacterium acnes (P. acnes) experiments, we found that SFPM has mild anti-bacterial effect (78% of bacteriosis), and in the case of 12.5 μL TTO addition, the inhibition ratio can reach approximately 98-99%. In animal model evaluation, we found that increasing the numbers of treatment day, the inhibition rates of P. acnes in the experimental group are significantly increased. Conclusively, we successfully develop a SFP-based membrane, which is potentially useful on the treatment of skin acne problem.
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