Research on Combining Photo-curable PCL with PEG-diacrylate and Chitosan to Fabricate Tissue Engineering Scaffold

• Main Authors: FREEMAN CHEN, 陳定閒
• Other Authors: Yih-Lin Cheng
• Format: Others
• Language: zh-TW
• Published: 2014
• Online Access: http://ndltd.ncl.edu.tw/handle/68306235285940594431

碩士 === 國立臺灣科技大學 === 機械工程系 === 102 === Tissue Engineering combines the principles and methods of engineering and life science to develop biological substitutes to restore, maintain and improve human tissue functions. Scaffold is one of the three key elements in Tissue Engineering. Layered manufacturing techniques, also known as Additive manufacturing (AM) processes, provide a great opportunity to fabricate 3D scaffolds without problems such as limited control of pore-size and restricted geometric shapes in traditional methods. The Biomedical Maskless Rapid Prototyping System developed in our previous research, utilizing the visible light to cure photo-curable PCL-type biodegradable material, has proven the feasibility of building 3D scaffolds with several layers. However, PCL is more hydrophobic. On the other hand, chitosan is great natural biomaterials with high biocompatibility and hydrophilic property. Therefore, in this research, we plan to add chitosan individually into the photo-curable PCL material system and utilize Biomedical Maskless Rapid Prototyping System to fabricate tissue engineering scaffolds. Tests will be conducted to understand the improvement of scaffold properties after addition of chitosan. The results has shown that adding Chitosan can effectively increase the hydrophilicity and biocompatibility. We find that addition of 10% Chitosan material composition has good hydrophilicity and precision. Also, the strength can be maintained at the required level. The other hand, cell culture also shows outstanding growth effect. In this study, we improve the shortcomings of the original hydrophobic by adding Chitosan, and we make the material has excellent biocompatibility that can be widely used in biomedical tissue engineering scaffolds.