The applicability of microtube array membrane module for bioreactor

• Main Authors: Wan-cheng Wu, 吳宛錚
• Other Authors: Ming-Chien Yang
• Format: Others
• Language: zh-TW
• Published: 2014
• Online Access: http://ndltd.ncl.edu.tw/handle/15592476873852883351

碩士 === 國立臺灣科技大學 === 材料科學與工程系 === 102 === In this study, PLLA was electrospun into microtube array membrane (MTAM). The resulting MTAM was examined using SEM and PMI to calculate average fiber diameter and pore size, using tensile test to determine mechanical strength, using TGA and DSC to measure pyrolysis temperature and thermal properties. The surface of MTAM was treated by oxygen plasma to improve fibroblasts (L929) attachment and growth, followd by using EDC/NHS coupling reaction to graft RGD peptide on MTAM surfuce, and employing Fourier transform infrared spectroscopy (FT-IR) to determine the amide group, and dyeing test to measure the graft density, and using contact angle to determine hydrophilic properties. The biocompatibility was evaluated with fibroblasts. The housing of the bioreactor was made of PMMA transparent tube with inner diameter of 16mm. The PLA joints were fabricated by 3D printing and bonded with epoxy resins. The cytocompatibility was evaluated with fibroblasts. The performance of the bioreactor was evaluated by passing gas was put through the lumens of the microtube, and fibroblasts were seeded in the MTAM surface. The results show that the proliferation of fibroblasts increased with the number of MTAM layers. Thus this demonstrated the feasibility of MTAM bioreactor for bioartificial liver.