| Summary: | 碩士 === 國立虎尾科技大學 === 生物科技研究所 === 102 === Royal jelly (RJ) has been shown to possess immunomodulatory activity, especially its protein components. However, almost nothing is known about influence of RJ fatty acids on the immune system. The anti-inflammatory effect of 10-hydroxy-2-decenoic acid (10-HDA), a major fatty acid in RJ was investigated. In this study, we studied the effect of 10-HDA on the cell viability and the release of important inflammatory cytokines from WiDr cell. To investigate the effect of 10-HDA, we treated WiDr human colon cancer cells with various concentration of 10-HDA. The higher dose (5 mM) of 10-HDA has cytotoxic effect on WiDr, it could directly kill the colon cancer cells. A lower dose of 10-HDA (2 and 3 mM), although non-cytotoxic, but it has significant inhibition of the pro-inflammatory cytokines. The cytokines that inhibited are interleukin (IL)-8, IL-1β, and tumor necrosis factor-alpha (TNF-α). It also significantly induces receptor antagonist cytokine (IL-1ra). 10-HDA inhibited the cell growth of WiDr in a dose-dependent manner. These data suggest that a non-toxic concentration of 10-HDA has a significant effect on the in vitro growth of WiDr cells, and may have value as a therapeutic immunomodulatory property.
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