Efficacy Study of Cysteine Containing Supplement in D-galactose Induced Aging Animal Model

• Main Authors: Laurensia Ananda, 林麗琴
• Other Authors: Wang, Shu Yin
• Format: Others
• Language: en_US
• Published: 2014
• Online Access: http://ndltd.ncl.edu.tw/handle/4y875a

碩士 === 中國文化大學 === 生物科技研究所 === 103 === Cysteine has been well-known as anti-aging amino acid. Cysteine may help to decrease the effects of aging, protect against heart disease and cancer, boost the immune system, promotes metabolism of fats and production of muscle tissue, healing after surgery, promote hair growth, and prevent hair loss. Some studies suggest that loss of youth, health and quality of life may be partly explained by a deficit in cysteine. The objective of this study is to evaluate the efficacy of cysteine, an anti-aging supporting anti-oxidant, on D-galactose induced aging in an experimental mouse model. Mice were divided into 6 groups, one control group and 5 groups of D-galactose induced aging mice (aging control group, low dose (81.25 mg/kg bw/day), regular dose (162.5 mg/kg bw/day, high dose (812.5 mg/kg bw/day) of cysteine, and one group was administrated by L-cysteine (50 mg/kg bw/day). We used subcutaneous injection of D-galactose (100 mg/kg bw/day) to induce aging in mice, cysteine and L-cysteine is administrated by gavage for 8 weeks. After 8 weeks the mice were sacrificed, tissue samples were analyzed for mean activities of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), glutathione-S-transferase (GST), glutathione reductase (GR). The concentration of reduced glutathione (GSH), malondialdehyde (MDA) and protein carbonyl (PC) were also determined. The histophatological study was performed in order to morphological conformation. The mean level of glutathione from liver was significantly lower in D-galactose control group (9.18 μg/mg protein) than in control group (15.41 μg/mg protein), and increasing after the administration of cysteine containing supplement (16.41; 14.41; 15.99 μg/mg protein). The enzymatic antioxidant activities (SOD, GST and GPx) were increased significantly by cysteine treatment. In addition cysteine repairs the damage of protein peroxidation by lowering the level of protein carbonyl in liver tissue. In conclusion, cysteine can be used as an antioxidant supplement by promoting the antioxidant in tissue and lowering the damage of protein.