Detection of bisphenol A using layer-by-layer anchoring of polyamidoamine dendrimer to gold electrodes

• Main Authors: Tseng, Chia-Wei, 曾家葳
• Other Authors: Su, Pi-Guey
• Format: Others
• Language: zh-TW
• Published: 2013
• Online Access: http://ndltd.ncl.edu.tw/handle/55857357797194556564

碩士 === 中國文化大學 === 化學系應用化學碩士班 === 102 === Novel amperometric bisphenol A (BPA) sensors were fabricated by layer-by-layer covalently bonding polyamidoamine (PAMAM) dendrimers onto the gold electrode on quartz crystal substrate using a peptide chemical protocol. Au electrodes was pretreated with 3-mercaptopropionic acid (MPA) and further reacted with generation 5 amine-terminated PAMAM dendrimer (G5-NH2) using N-(3-dimethylaminopropyl)-N-ethylcarbodiimide hydrochloride (EDC) as peptide coupling reagent. The prepared BPA-sensing film was characterized by atomic force microscopy (AFM) and electrochemical impedance spectroscopy (EIS). The effects of the intermediate layer, the generations (sizes) of PAMAM dendrimer and the concentration of PAMAM dendrimer on the BPA sensing properties (response) of the sensors were investigated to optimize the fabrication of the modified electrodes. Additionally, factors influencing the detection processes were optimized. The BPA sensor based on G5-NH2-MPA/Au electrode exhibited a high response, an acceptable linearity, low detection limit and good long-term stability.