| Summary: | 碩士 === 靜宜大學 === 食品營養學系 === 102 === Corchorus capsularis , also known as jute or jews mallow, is atropical and subtropical crop. Not only providing high fiber for rope making, the leaves of jute is rich in mucilage that is good for soup traditionally, one of famous local snack. The purposes of this study are to investigate the antioxidant and anti-inflammatory activity of jute’s extracts, andto compare the physicochemical properties of crude polysaccharides among the extracts. The antioxidant and anti-inflammatory activity of the extracts of jute from different ratios of ethanol were also determined. Moreover, three extracted methods, including cold water (CCP), hot water (CHP) and ultrasonic (CUP) extraction, were usedto determine the yield and chemical compositions of crude polysaccharides. The monosaccharide compositions, functional groups, molecular weight distribution and viscosity of polysaccharides were also analyzed in this study. The results indicated that theprimaryproximate compositions of the leaves of juteare carbohydrate and protein, up to 25.13% in protein. The potassium is the most abundant mineral, up to 623.2 mg %, following by calcium, magnesium, sodium and iron. No significant difference was observed in the yield of crude polysaccharide among three extracts. Antioxidant activity assay revealed that the Ce50 extracts obtained the best ferrous ion chelating ability. The chelating effect reached 91% as the concentration of extracted was at 3 mg/ml. The best ABTS radicals and DPPH free radical scavenging capacity of extracts were obtained in Ce95 method up to 98% and 89 %, respectively, in both 50 μg/mL and 5 mg/mL. The higher concentration of crude extracts of jute the lower cell viability were obtained on the growth of Raw264.7 cells. As the concentration of extracts increased to 500μM/mL, cell viability of Raw264.7 decreased to 20% on the Ce50 and Ce95 extracts except Cw extracts. The inhibition of NO production increases of concentration of jute’s extracts up to 50μM/mL. The highest inhibition with the increased was found in Ce95 extracts, up to 68.3%. There finding indicated that Ce95 extracts may have potential application in the antioxidant and anti-inflammatory activity of health foods development. Comparison of cold water, hot water, and ultrasonic extraction in the yield of extracts, the highest yield of extracts was found in ultrasonic extraction, up to 25%, which more than twice of hot water and cold water in the yield. The same monosaccharide compositions in three crude polysaccharides demonstrated extracted method did not influence the compositions of monosaccharide. Glucose is the primary monosaccharide, following by galactose, arabinose, rhamnose and xylose. Highuronic acid content was identified in crude polysaccharides of jute resulted in an acidic polysaccharides. The CHP extracted method obtained the higher total carbohydrate and uronic acid in the crude polysaccharides, 42% and 61 mg/g, respectively. The Fourier transform infrared spectra revealed and indicated the general characteristicabsorption peaks of three polysaccharides were similar. Non-Newtonian fluidbehavior was observed in three polysaccharides solutions which indicated the viscosity increasing with the increases of concentration of polysaccharides, but decreasing with the increases of temperature. The highest viscosity was located at CHP extracted solution.
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