The Development of Voltage-Assisted Liquid-Phase Microextraction and its Application

• Main Authors: Mi-Hung Wang, 王米宏
• Other Authors: Shu-Ping Wang
• Format: Others
• Language: zh-TW
• Published: 2014
• Online Access: http://ndltd.ncl.edu.tw/handle/79514767188708586487

博士 === 靜宜大學 === 應用化學系 === 102 === A voltage-assisted liquid-phase micoextraction (VA-LPME) combined with LC-MS was developed. We expected that VA-LPME could reduce amount of organic solvent and volume of sample, shorten extraction time and improve repeatability. In VA-LPME, some parameters, such as pH of donor and acceptor phase, stirring rate, organic solvent, equilibrium time and voltage-applied were also evaluated. After optimization, VA-LPME applied in determination of quinolones and sulfonamides in water samples. In quinolones, the LOD ranged from 1.5 to 2.0 ng/mL, enrichment factors are 18.3 to 38.8 folds, and recoveries are 90.8 to 104.9%; in sulfonamides, the LOD ranged from 1.4 to 8.5 ng/mL, enrichment factors are 16.0 to 104.3 folds, recoveries are 84.9 to 96.9%; and RSDs are smaller than 10.7%. The LOQs are smaller than the EC50 (19-200 ng/mL, quinolones; 146 ng/mL, sulfonamides), and it expresses VA-LPME can applied in determination of water samples. VA-LPME solved some problems, for example, sample amount needed (5-mL sample volume only), trace amount of solvent needed (25μL), shorter extraction time (20 min), acceptable reproducibility, and good recovery. Clearly,VA-LPME is a simple, fast and eco-friendly extraction technique.