| Summary: | 碩士 === 東吳大學 === 微生物學系 === 102 === Nonylphenol(NP) is an environmental endocrine disruptor. This sort of compound was detected around the polluted soil in the river. In this study, we investigate the effect of removing nonylphenol by Eisenia fetida in the compost substrate; it takes two kinds of material: compost and dehydrate active sludge. In the meantime, we try to find out the best condition of nonylphenol removal, and observe the survival rate of the earthworm. Finally, we use the NGS-pyrosequencing to analyze the microbial community structure and discuss the relationship between bacterium and toxin removal. We compare the results of the samples which contain living earthworms as well as the experiment control, and the result showed the removal of nonylphenol in the sample which included living earthworms is better. On the other hand, we know the substrate of samples were contained sludge which are not only with the same good effect of removing toxins, but also make earthworms grow very well. Therefore, we set an experiment about using earthworm cast to remove nonylphenol, and the result showed that the concentration of nonylphenol decreases with the same rate in adding no matter the pure cast or the mixed cast with soil (1:1) . But so far, the process of mechanism is uncertain. Even so it’s still a key point about development of earthworm cast as a soil amendment. The result of microbial community structure shows those main degraders aren’t the Sphingomonas spp. and Pseudomonas spp. As previous study, it might be Bacillus spp. because of the proportion change between nonylphenol and Bacillus spp. In this case, the part of nonylphenol decreased and the portion of Bacillus spp. increase significantly.This information is compiled to assess the final day of nonylphenol pollution as bioremediation feasibility of reference.
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