| Summary: | 碩士 === 東海大學 === 化學工程與材料工程學系 === 102 === In drug delivery system, the biocompatibility of a carrier is really important, and the morphology and dispersion of the carrier particles are considered as the major influence to the biocompatibility. Therefore, we will establish an optimum the condition to synthesize well-dispersed spherical SBA-15 in this study and investigate. The protein adsorption and release as well. Meanwhile, we will improve the protein immobilization by tailoring the surface chemistry of SBA-15.
Based on the results from the synthesized SBA-15, stirring while adding TEOS could cause the interactions between micelles, in which prpmote the severe aggregation of SBA-15, therefore a settling process was recommended. The concentration of hydrochloric acid could dramatically influence the ionic strength of the reaction solution, and both the hydrophobic and hydrophilic chain of triblock copolymer P123. We found an appropriate concentration (2 M HCl) could increase zeta-potential of SBA-15 and well-dispersed spherical SBA-15 was obtained. However the higher hydrogen concentration (3 M HCl) caused the fast condensation polymerization, and resulted in a serious aggergation. Besides adding swelling-agent TMB can change the micelle size, then to control the morphology and pore size distribution of SBA-15, which dependent on the concentrations of hydrochloric acid and salts used.
This study has also shown that the charges between the carrier and protein would have a great influence on protein adsorption by carrier. For example, when the carrier and lysozyme displaced opposite charge on their surface, the amount of protein adsorbed will be 3 times higher than that for those for same type of charges. Because of the significant electronstatic force between carrier and protein, we can clearly observe the slow drug release phenomenon based on the lysozyme releasing test.
Adsorptions of Bovine serum albumin (BSA) at pH 5 and lysozyme at pH 8 on SBA-15 functionalized with chloromethyl (CM), octyl (Oc) or aminopropyl (AP) groups were investigated and were compared with those without modification. The results have shown that the adsorption performance, including adsorption capacity and adsorption rates can be tailored by incorporating a specific functional group and its modification efficiency. Therefore, we have confirmed the feasibility for our synthesized spherical SBA-15 (or functionalized one) as a carrier for macromolecular drugs delievery system.
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