Preparation of Fe@Au Core-Shell Nanoparticles for Thermotherapy and Drug Control Release

• Main Authors: Hui-Ting Shih, 施惠婷
• Other Authors: Ren-Jei Chung
• Format: Others
• Language: zh-TW
• Published: 2014
• Online Access: http://ndltd.ncl.edu.tw/handle/3v834r

碩士 === 國立臺北科技大學 === 化學工程研究所 === 102 === This study was to prepare Fe@Au core-shell nanoparticles (Fe@Au) by microemulsion methods. The products were grafted with the anticancer drug methorexate (MTX) to become multifunctional magnetic nanocomposites applying to magnetic resonance imaging, magnetic guiding, magnetic hyperthermia treatment and drug control release. Specific textured structure was further fabricated onto the nanoparticles to enhance optical properties. The crystalline structure was determined using XRD to verify the phases of iron and gold. The molar ratio of Fe to Au was 1:1 through EDS analyzed. According to the results of SQUID tests, the Fe@Au nanoparticles were superparamagnetic at room temperature and the saturated magnetization were 29 emu/g under 10000 Oe. A high-frequency wave generator was used to test the magnetic hyperthermia property. The temperature of 10 mg/mL Fe@Au solution was increased from room temperature to 48.5oC in 20 minutes. Results of in vitro test showed that a dose below 500 μg/mL was not cytotoxic to L929 cell. The UV-VIS spectra presented an absorption peak at 310 nm, which confirmed the successful surface grafting of MTX. For the textured Fe@Au core-shell nanoparticles (tFe@Au), they presented a cobble stone shape with specific ridges through TEM and SEM observation. The molar ratio of Fe to Au was 1:3 through EDX analysis. The results of UV-VIS spectroscopy showed that tFe@Au had absorption peaks at 540 nm, and between 750 nm and 800 nm. We conclude that the magnetic nanocomposites are able to be applied for cancer hyperthermia treatment.