Study of Antrodia camphorata combined with aspirin in transient MCAO

• Main Authors: Meng-Che Yu, 余孟哲
• Other Authors: 馮琮涵
• Format: Others
• Language: zh-TW
• Published: 2014
• Online Access: http://ndltd.ncl.edu.tw/handle/7729dg

碩士 === 臺北醫學大學 === 醫學科學研究所 === 102 === Stroke is the third leading cause of mortality in Taiwan. So far, there are no better medicine to treat stroke. Therefore, this disease always results a heavy social burden in taking care of patient.Antrodia camphorata, a native species of fungi in Taiwan,is considered as a dietary supplement due to the enrichment of triterpenoid. It has been widely used in liver disease or even cancertreatment. Recently, some studies also indicated that Antrodia camphorata maybe beneficial on the prevention and treatment of stroke and cardiovascular diseases,but the mechanism and possible side effect are not well known yet.Aspirin is the most commonly used drug in clinic for the treatment or prevention of stroke, but many studies indicated aspirin has a higher risk of hemorrhage. Thus, to drug in the remedy of stroke with better effectiveness and less adverse effect is an important issue of stroke research.On the other hand, there are many patients in Taiwan concurrently takewestern medicine and health food to treat and prevent of stroke.Therefore, in the present study, wetreated aspirin combined with Antrodia camphoratato investigate their protective efficacy after ischemic stroke. In addition, we alsoestimate hemorrhagic side effects. In this study, our results showed that treatment of Antrodia camphorata (0.25 or 0.75 g/kg) in the absence or presence of aspirin (5 mg/kg) by oral gavages daily after transient MCAO for two months markedly reduced infarct size and improved neurologic functions.We further observed several proteins, including iNOS, HO-1, Bax, and caspase-3, which are associated with brain injury. Our data revealed that Antrodia camphorate markedly decrease the expression of these proteins. Antrodia camphorata In addition, treatment of Antrodia camphorata combined with aspirin or not didn’t have side effects of hemorrhage such as intracerebral hemorrhage (ICH), subarachnoid hemorrhage (SAH), gastric bleeding (GB), and prothrombin time (PT). In conclusion, this study demonstrates that given alone Antrodia camphorata (0.25 and 0.75 g / kg), aspirin (5 mg / kg) or with both has a potent neuroprotective activity. This activity is mediated, at least in part, through inhibition of apoptosis, oxidative stress, resulting in a reduction of infarct volume and improvement in neurobehavior in rats with focal cerebral ischemia without hemorrhaging side effects. Therefore, Antrodia camphorata may represent high therapeutic potential and safe for treatment of ischemic stroke.