| Summary: | 碩士 === 臺北醫學大學 === 藥學系(碩博士班) === 102 === In this study, we investigated the effect of ginsenoside Rg1 (GS Rg1) and N-acetylcysteine (NAC) on the concentration of methylglyoxal (MG) and D-lactate and evaluated the MG and D-lactate as indicators in cisplatin-induced nephrotoxicity kidney tissues.
We divided 30 six-week-old female BALB/c mice into five groups. Then, we used cisplatin (5 mg/kg/day) intraperitoneally to induce nephrotoxicity for 5 day except for the Normal group at the 6th day. Besides, the mice were orally administered GS Rg1 5 mg/kg/day, NAC 450 mg/kg/day and GS Rg1+NAC for 10 days, respectively. At the 10th day, the mice were sacrificed and the kidney tissue was homogenized and centrifuged. The supernatants were derivatized with fluorescent reagent 4-nitro-7-piperazino-2,1,3-benzoxadiazole (NBD-PZ) or 5,6-diamino-2,4-
dihydroxypyrimidine (DDP) for D-lactate and MG determination.
The results showed that the levels of MG and D-lactate increased significantly in the Control group compared with the Normal group. (MG: 64.6 ± 22.0 vs. 284.1 ± 327.3 μg/mg, D-lactate: 11.22 ± 1.52 vs. 20.74 ± 16.21μmol/g, p < 0.01) Administration of GS Rg1, NAC or GS Rg1+NAC revealed a decreasing tendency of both the levels of MG and D-lactate. Particularly, the D-lactate level significantly decreased to 10.96 ± 2.85 μmol/g in the GS Rg1+NAC group. In addition, it had good co-relationship between the D-lactate level and the histological outcome. As a result, we considered that the D-lactate level reflects the tissue injuries and the D-lactate level is a better indicator to tissue injuries than MG.
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