Effects of ginsenoside Rg1 and N-acetylcysteine on methylglyoxal and D-lactate in cisplatin-induced nephrotoxicity kidney tissues in inbred mice
碩士 === 臺北醫學大學 === 藥學系(碩博士班) === 102 === In this study, we investigated the effect of ginsenoside Rg1 (GS Rg1) and N-acetylcysteine (NAC) on the concentration of methylglyoxal (MG) and D-lactate and evaluated the MG and D-lactate as indicators in cisplatin-induced nephrotoxicity kidney tissues. We di...
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ndltd-TW-102TMC055510182019-08-03T15:50:51Z http://ndltd.ncl.edu.tw/handle/p97r7w Effects of ginsenoside Rg1 and N-acetylcysteine on methylglyoxal and D-lactate in cisplatin-induced nephrotoxicity kidney tissues in inbred mice 人參皂苷Rg1與N-乙醯半胱胺酸對於順氯氨鉑引發腎毒性之純系小鼠腎組織中甲基乙二醛及D-乳酸的影響 Mu-Cheng Tsai 蔡牧承 碩士 臺北醫學大學 藥學系(碩博士班) 102 In this study, we investigated the effect of ginsenoside Rg1 (GS Rg1) and N-acetylcysteine (NAC) on the concentration of methylglyoxal (MG) and D-lactate and evaluated the MG and D-lactate as indicators in cisplatin-induced nephrotoxicity kidney tissues. We divided 30 six-week-old female BALB/c mice into five groups. Then, we used cisplatin (5 mg/kg/day) intraperitoneally to induce nephrotoxicity for 5 day except for the Normal group at the 6th day. Besides, the mice were orally administered GS Rg1 5 mg/kg/day, NAC 450 mg/kg/day and GS Rg1+NAC for 10 days, respectively. At the 10th day, the mice were sacrificed and the kidney tissue was homogenized and centrifuged. The supernatants were derivatized with fluorescent reagent 4-nitro-7-piperazino-2,1,3-benzoxadiazole (NBD-PZ) or 5,6-diamino-2,4- dihydroxypyrimidine (DDP) for D-lactate and MG determination. The results showed that the levels of MG and D-lactate increased significantly in the Control group compared with the Normal group. (MG: 64.6 ± 22.0 vs. 284.1 ± 327.3 μg/mg, D-lactate: 11.22 ± 1.52 vs. 20.74 ± 16.21μmol/g, p < 0.01) Administration of GS Rg1, NAC or GS Rg1+NAC revealed a decreasing tendency of both the levels of MG and D-lactate. Particularly, the D-lactate level significantly decreased to 10.96 ± 2.85 μmol/g in the GS Rg1+NAC group. In addition, it had good co-relationship between the D-lactate level and the histological outcome. As a result, we considered that the D-lactate level reflects the tissue injuries and the D-lactate level is a better indicator to tissue injuries than MG. 林本元 2014 學位論文 ; thesis 119 zh-TW |
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碩士 === 臺北醫學大學 === 藥學系(碩博士班) === 102 === In this study, we investigated the effect of ginsenoside Rg1 (GS Rg1) and N-acetylcysteine (NAC) on the concentration of methylglyoxal (MG) and D-lactate and evaluated the MG and D-lactate as indicators in cisplatin-induced nephrotoxicity kidney tissues.
We divided 30 six-week-old female BALB/c mice into five groups. Then, we used cisplatin (5 mg/kg/day) intraperitoneally to induce nephrotoxicity for 5 day except for the Normal group at the 6th day. Besides, the mice were orally administered GS Rg1 5 mg/kg/day, NAC 450 mg/kg/day and GS Rg1+NAC for 10 days, respectively. At the 10th day, the mice were sacrificed and the kidney tissue was homogenized and centrifuged. The supernatants were derivatized with fluorescent reagent 4-nitro-7-piperazino-2,1,3-benzoxadiazole (NBD-PZ) or 5,6-diamino-2,4-
dihydroxypyrimidine (DDP) for D-lactate and MG determination.
The results showed that the levels of MG and D-lactate increased significantly in the Control group compared with the Normal group. (MG: 64.6 ± 22.0 vs. 284.1 ± 327.3 μg/mg, D-lactate: 11.22 ± 1.52 vs. 20.74 ± 16.21μmol/g, p < 0.01) Administration of GS Rg1, NAC or GS Rg1+NAC revealed a decreasing tendency of both the levels of MG and D-lactate. Particularly, the D-lactate level significantly decreased to 10.96 ± 2.85 μmol/g in the GS Rg1+NAC group. In addition, it had good co-relationship between the D-lactate level and the histological outcome. As a result, we considered that the D-lactate level reflects the tissue injuries and the D-lactate level is a better indicator to tissue injuries than MG.
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author2 |
林本元 |
author_facet |
林本元 Mu-Cheng Tsai 蔡牧承 |
author |
Mu-Cheng Tsai 蔡牧承 |
spellingShingle |
Mu-Cheng Tsai 蔡牧承 Effects of ginsenoside Rg1 and N-acetylcysteine on methylglyoxal and D-lactate in cisplatin-induced nephrotoxicity kidney tissues in inbred mice |
author_sort |
Mu-Cheng Tsai |
title |
Effects of ginsenoside Rg1 and N-acetylcysteine on methylglyoxal and D-lactate in cisplatin-induced nephrotoxicity kidney tissues in inbred mice |
title_short |
Effects of ginsenoside Rg1 and N-acetylcysteine on methylglyoxal and D-lactate in cisplatin-induced nephrotoxicity kidney tissues in inbred mice |
title_full |
Effects of ginsenoside Rg1 and N-acetylcysteine on methylglyoxal and D-lactate in cisplatin-induced nephrotoxicity kidney tissues in inbred mice |
title_fullStr |
Effects of ginsenoside Rg1 and N-acetylcysteine on methylglyoxal and D-lactate in cisplatin-induced nephrotoxicity kidney tissues in inbred mice |
title_full_unstemmed |
Effects of ginsenoside Rg1 and N-acetylcysteine on methylglyoxal and D-lactate in cisplatin-induced nephrotoxicity kidney tissues in inbred mice |
title_sort |
effects of ginsenoside rg1 and n-acetylcysteine on methylglyoxal and d-lactate in cisplatin-induced nephrotoxicity kidney tissues in inbred mice |
publishDate |
2014 |
url |
http://ndltd.ncl.edu.tw/handle/p97r7w |
work_keys_str_mv |
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