Cellulase production by Aspergillus niger under solid-state fermentation and applied in cellulosic ethanol production
碩士 === 大同大學 === 生物工程學系(所) === 102 === Wheat bran was used as substrate for Aspergillus niger TU-17 to produce cellulase. A circular bamboo steamer (9 cm ID) was used in the solid culturing process under the following culture condition: initial substrate pH 4.8; temperature 30°C; 40% moisture content...
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ndltd-TW-102TTU051060442019-05-15T21:32:55Z http://ndltd.ncl.edu.tw/handle/38aat6 Cellulase production by Aspergillus niger under solid-state fermentation and applied in cellulosic ethanol production 以Aspergillus niger固態發酵生產之Cellulase進行生產纖維素酒精之研究 Yuan-chi Chen 陳淵琪 碩士 大同大學 生物工程學系(所) 102 Wheat bran was used as substrate for Aspergillus niger TU-17 to produce cellulase. A circular bamboo steamer (9 cm ID) was used in the solid culturing process under the following culture condition: initial substrate pH 4.8; temperature 30°C; 40% moisture content, incubation time 6 days. 10% (v/w) of liquid seed culture was inoculated to the wheat bran. The maximum cellulase activity was 26 FPU/g-DS on the 4th day, and 刍-glucosidase 130 U/g-DS, CMCase 62.3 U/g-DS, xylanase 250.8 U/g-DS were concurrently obtained after 4-6 days of cultivation. The cellulase was extracted from the solid substrate and concentrated, the specific activity of FPU, 刍-glucosidase, CMCase, and xylanase were 17.7 U/mg, 87.8 U/mg, 42.1 U/mg, 169.5 U/mg respectively. The individual specific activity of cellulase in this study was compared to some commercial cellulases. The cellulase of this study was employed to produce ethanol from 脉-cellulose. This process was carried out by simultaneous saccharification and fermentation (SSF) using a thermotolerant Kluyveromyces marxianus at 40 牵C. A 112.5 FPU cellulase from Asp. niger was added to 100 ml medium with 7.5, 5, and 2.5 g 脉-cellulose (Sigma). The theoretical yields of ethanol production were 56, 42, 24 % from 2.5, 5.0, and 7.5% 脉-cellulose respectively. The supplement of commercial 刍-glucosidase increased the ethanol conversion yield to 82 % in 7.5% 脉-cellulose. The results indicated that cellulase produced from Asp. niger in this study can be used in ethanol production using the lignocellulose of agricultural wastes. Kow-jen Duan 段國仁 2014 學位論文 ; thesis 93 zh-TW |
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碩士 === 大同大學 === 生物工程學系(所) === 102 === Wheat bran was used as substrate for Aspergillus niger TU-17 to produce cellulase. A circular bamboo steamer (9 cm ID) was used in the solid culturing process under the following culture condition: initial substrate pH 4.8; temperature 30°C; 40% moisture content, incubation time 6 days. 10% (v/w) of liquid seed culture was inoculated to the wheat bran. The maximum cellulase activity was 26 FPU/g-DS on the 4th day, and 刍-glucosidase 130 U/g-DS, CMCase 62.3 U/g-DS, xylanase 250.8 U/g-DS were concurrently obtained after 4-6 days of cultivation. The cellulase was extracted from the solid substrate and concentrated, the specific activity of FPU, 刍-glucosidase, CMCase, and xylanase were 17.7 U/mg, 87.8 U/mg, 42.1 U/mg, 169.5 U/mg respectively. The individual specific activity of cellulase in this study was compared to some commercial cellulases.
The cellulase of this study was employed to produce ethanol from 脉-cellulose. This process was carried out by simultaneous saccharification and fermentation (SSF) using a thermotolerant Kluyveromyces marxianus at 40 牵C. A 112.5 FPU cellulase from Asp. niger was added to 100 ml medium with 7.5, 5, and 2.5 g 脉-cellulose (Sigma). The theoretical yields of ethanol production were 56, 42, 24 % from 2.5, 5.0, and 7.5% 脉-cellulose respectively. The supplement of commercial 刍-glucosidase increased the ethanol conversion yield to 82 % in 7.5% 脉-cellulose. The results indicated that cellulase produced from Asp. niger in this study can be used in ethanol production using the lignocellulose of agricultural wastes.
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author2 |
Kow-jen Duan |
author_facet |
Kow-jen Duan Yuan-chi Chen 陳淵琪 |
author |
Yuan-chi Chen 陳淵琪 |
spellingShingle |
Yuan-chi Chen 陳淵琪 Cellulase production by Aspergillus niger under solid-state fermentation and applied in cellulosic ethanol production |
author_sort |
Yuan-chi Chen |
title |
Cellulase production by Aspergillus niger under solid-state fermentation and applied in cellulosic ethanol production |
title_short |
Cellulase production by Aspergillus niger under solid-state fermentation and applied in cellulosic ethanol production |
title_full |
Cellulase production by Aspergillus niger under solid-state fermentation and applied in cellulosic ethanol production |
title_fullStr |
Cellulase production by Aspergillus niger under solid-state fermentation and applied in cellulosic ethanol production |
title_full_unstemmed |
Cellulase production by Aspergillus niger under solid-state fermentation and applied in cellulosic ethanol production |
title_sort |
cellulase production by aspergillus niger under solid-state fermentation and applied in cellulosic ethanol production |
publishDate |
2014 |
url |
http://ndltd.ncl.edu.tw/handle/38aat6 |
work_keys_str_mv |
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