The role of miR-122 in macrophage functions
碩士 === 國立陽明大學 === 微生物及免疫學研究所 === 102 === MicroRNAs (miRNAs) are endogenously non-coding RNAs involved in posttranscriptional gene repression. Although miR-122 is considered as a dominant liver-specific miRNA, previous studies revealed that Mir122-null mice have increased numbers of Kupffer cells, mo...
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ndltd-TW-102YM0053800232015-10-13T23:50:23Z http://ndltd.ncl.edu.tw/handle/47868227921956524374 The role of miR-122 in macrophage functions miR-122對巨噬細胞功能的影響 Yi-Ru Su 蘇意茹 碩士 國立陽明大學 微生物及免疫學研究所 102 MicroRNAs (miRNAs) are endogenously non-coding RNAs involved in posttranscriptional gene repression. Although miR-122 is considered as a dominant liver-specific miRNA, previous studies revealed that Mir122-null mice have increased numbers of Kupffer cells, monocytes, and neutrophils in the liver. Our previous results showed that injection of LPS in mice resulted in a significantly higher serum IL-6 in the Mir122-/- mice than that in wild type (WT) mice. We hypothesize that macrophages may express Mir122, and miR-122 may regulate macrophage functions. Here we confirmed that serum IL-6 was significantly higher in Mir122-/- mice than in WT controls after injection of LPS. However, there were no differences in the cell numbers and in the percentages of peritoneal macrophages between Mir122-/- and WT mice. A low level of miR-122 was detected in the peritoneal macrophages. The addition of LPS stimulated higher IL-6 production by purified peritoneal Mir122-/- macrophages. We observed that the phagocytosis ability was similar between Mir122-/- and WT macrophages. We also examined markers for M1 and M2 macrophages, and our analysis showed that Mir122-deficent macrophages were not biased toward M1 polarization. Since NF-κB is one of the major transcription factors regulating the expression of inflammatory cytokines, we examined the effect of miR-122 on NF-κB activity by reporter assay. We found that Mir122-/- macrophages had higher NF-κB activity than WT macorphages. Several genes in the NF-κB pathway contain miR-122 target sites and we identified that they are true targets by reporter assay. Consistent with this observation, the expreesion of two target genes was higher in the Mir122-/- macrophages than the expression of those in WT. In summary, our findings suggest that miR-122 may negatively regulate the NF-κB pathway and help fine-tune the immune response. Chuen-Miin Leu 呂春敏 2014 學位論文 ; thesis 60 zh-TW |
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碩士 === 國立陽明大學 === 微生物及免疫學研究所 === 102 === MicroRNAs (miRNAs) are endogenously non-coding RNAs involved in posttranscriptional gene repression. Although miR-122 is considered as a dominant liver-specific miRNA, previous studies revealed that Mir122-null mice have increased numbers of Kupffer cells, monocytes, and neutrophils in the liver. Our previous results showed that injection of LPS in mice resulted in a significantly higher serum IL-6 in the Mir122-/- mice than that in wild type (WT) mice. We hypothesize that macrophages may express Mir122, and miR-122 may regulate macrophage functions. Here we confirmed that serum IL-6 was significantly higher in Mir122-/- mice than in WT controls after injection of LPS. However, there were no differences in the cell numbers and in the percentages of peritoneal macrophages between Mir122-/- and WT mice. A low level of miR-122 was detected in the peritoneal macrophages. The addition of LPS stimulated higher IL-6 production by purified peritoneal Mir122-/- macrophages. We observed that the phagocytosis ability was similar between Mir122-/- and WT macrophages. We also examined markers for M1 and M2 macrophages, and our analysis showed that Mir122-deficent macrophages were not biased toward M1 polarization. Since NF-κB is one of the major transcription factors regulating the expression of inflammatory cytokines, we examined the effect of miR-122 on NF-κB activity by reporter assay. We found that Mir122-/- macrophages had higher NF-κB activity than WT macorphages. Several genes in the NF-κB pathway contain miR-122 target sites and we identified that they are true targets by reporter assay. Consistent with this observation, the expreesion of two target genes was higher in the Mir122-/- macrophages than the expression of those in WT. In summary, our findings suggest that miR-122 may negatively regulate the NF-κB pathway and help fine-tune the immune response.
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author2 |
Chuen-Miin Leu |
author_facet |
Chuen-Miin Leu Yi-Ru Su 蘇意茹 |
author |
Yi-Ru Su 蘇意茹 |
spellingShingle |
Yi-Ru Su 蘇意茹 The role of miR-122 in macrophage functions |
author_sort |
Yi-Ru Su |
title |
The role of miR-122 in macrophage functions |
title_short |
The role of miR-122 in macrophage functions |
title_full |
The role of miR-122 in macrophage functions |
title_fullStr |
The role of miR-122 in macrophage functions |
title_full_unstemmed |
The role of miR-122 in macrophage functions |
title_sort |
role of mir-122 in macrophage functions |
publishDate |
2014 |
url |
http://ndltd.ncl.edu.tw/handle/47868227921956524374 |
work_keys_str_mv |
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