| Summary: | 碩士 === 國立雲林科技大學 === 化學工程與材料工程系 === 102 === The physicochemical characteristics of liposomes composed of hydrogenated soybean phosphatidylcholine (HSPC), egg phosphatidylcholine (EPC), and 1-hexadecyl- 3-methylimidazolium ionic liquids (C16ILs) were studied by dynamic light scattering (DLS), transmission electron microscopy (TEM), fluorescence polarization, differential scanning calorimeter (DSC), Langmuir trough and fluorescence microscopy. In mixed HSPC/EPC/C16ILs bilayer at the various molar fractions, the incorporation of increasing quantities of EPC progressively increased intermolecular fluidity, that progressively reduced the temperature and the enthalpy of the gel-to-liquid crystalline transition. Adding C16MIMPF6 to mixed HSPC/EPC bilayer could reduced membrane fluidity. Furthermore, DNA-cationic liposomes complex were mixed at various N/P ratios. With N/P ratio decreasing, the membrane fluidity rising of mixed HSPC/EPC/C16ILs (9/0/1, 6.3/2.7/1 and 0/9/1 molar ratio), that increased enthalpy. Especially, with N/P ratio decreasing, the temperature of phase transition of mixed HSPC/EPC/C16MIMPF6 (9/0/1, 6.3/2.7/1 and 0/9/1 molar ratio) bilayer increased at the various molar fractions. In addition, the adsorption of DNA onto the mixed HSPC/EPC/C16ILs (9/0/1, 6.3/2.7/1 and 0/9/1 molar ratio) monolayers isotherms with high collapse and shifted toward the right. DNA molecules could partially penetrate into a monolayer at low surface pressure, through lateral pressure were compressed to reach high surface pressure, that DNA molecules were squeezed from the interface and induced part of molecules into the water subphase.
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