Bio-dehydrogenation Kinetics of Methyltestosterone Reaction in a Biphasic Reactor

• Main Authors: Chia-Yun Lai, 賴家藝
• Other Authors: Ruey-Shin Juang
• Format: Others
• Language: zh-TW
• Online Access: http://ndltd.ncl.edu.tw/handle/73875906424880649361

碩士 === 元智大學 === 化學工程與材料科學學系 === 102 === A two-phase bioreactor was conducted to enzymatically catalyze methyl-testosterone though a dehydrogenation reaction. Several conditions included loading concentration of methyl-testosterone, menadione, surfactant and wet biomass were investigated. Dehydrogenase was extracted from the fermentation broth. The influences of optimal solvents upon yield, specific enzyme activities were discussed. In addition, the effects of agitation intensity on enzymatic reaction were further studied in respect of enzyme kinetics. The results of solvent screening indicated toluene is an optimal solvent for using in such bioreactor where presented higher biodegradable and biocompatible capacities. Furthermore, organic-aqueous two phases bioreactor at volume ratio of 30% applied in the scale up experiments demonstrated that specific enzyme activities were key factor to achieve high yield. Operating conditions for scale-up reaction were described as following: 30 oC, 200rpm, the aqueous phase (70% v / v) - phosphate buffer solution (pH7.2) and an organic phase (30% v / v) - toluene, menadione change interface active agent, the concentration of wet bacterial enzyme kinetics and methyl testosterone were discussed. The results indicated that the 0.2 g / L menadione, 1.0 g / L of surfactant, 5.0 g / L and the cell concentration of 10 g / L methyl-estosterone had the highest yields; the substrate concentration and reaction rate for the kinetic diagram, when the working volume of 20 and 40 mL volume effect results in a greater impact; while working volume of 80mL size of the specific activity of the enzyme places affect the results.