| Summary: | 碩士 === 國立中正大學 === 分子生物研究所 === 103 === Glioblastoma multiforme (GBM) has universally poor prognosis that is thought to be related to cellular invasion into normal tissue, making localized treatments like surgery and radiation insufficient to treat the total burden of disease. Recently, stem cells have been found offering much promise as delivery vehicles for brain tumor therapy. Our previous study had confirmed the tumor-tropic migratory ability of human adipose-derived stem cells (hASC) and investigated the efficiency of hASC to deliver replicating retrovirus vector (RRV) into glioma cells in vitro and in vivo. This in vivo experiment will be repeated for validation. Our previous study also had determined the therapeutic effects of hASC-ACE-CD (RRV expressing the yeast cytosine deaminase suicide gene) by MTS assay and an orthotopic mouse model of human glioma after treatment with prodrug 5-FC. The therapeutic effect can also delay the onset of animals. To further validate this result, we will repeat this in vivo experiment, too. In order to more intuitively observe the changes in tumor size, we constructed a lentiviral vector containing near infrared fluorescent protein (iRFP) and transduced the viral vector into U87 cells followed by subcutaneous inoculation of the cells in nude mice. Then, we used the Fluorescence Molecular Tomography (FMT) to detect the iRFP signals for observation of the tumor growth and to track the effectiveness of treatment. We wish the use of hASC as the vehicle for dispersing RRV may provide a viable strategy for glioma therapy.
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