| Summary: | 碩士 === 長榮大學 === 醫學研究所 === 103 === Pogostemon cablin (PC) is a plant found in traditional Chinese medicine, traditionally used to treat the common cold, nausea, diarrhea, headaches, and fevers. Water extract from Pogostemon cablin (WEPC) has also been shown to induce apoptosis in human leukemia cells. One of its active ingredients in PC is patchouli alcohol (PA), which has been shown to inhibit cell proliferation of colon cancer cell lines HCT116 and SW480. This study investigated the influence of patchouli alcohol (PA), on human leukemia cells. PA has been shown to possess neuroprotective, anti-influenza and anti-inflammatory activities; however, the mechanisms underlying these effects have yet to be elucidated. This study sought to identify the mechanisms of PC extracts with regard to the treatment of human acute myeloid leukemia (AML) cell line U937 and the chronic myelogenous leukemia (CML) cell line K562. We focused on the anti-tumor properties of WEPC and PA in vitro in halting abnormal cell proliferation. Cell cycle progression and DNA content were analyzed using flow cytometry. Gene expression of cyclins/CDKs and apoptosis in Bcl-2 cell cycles were analyzed using PCR arrays, and real-time PCR. SDS-PAGE and western blotting analysis were used to identify the proteins associated with various cell cycles and apoptosis.
The results of caspase-3 activity and mitochondrial membrane potential (ΔѰm) demonstrate that WEPC and PA possess anticarcinogenic mechanisms other than the inhibition of cell proliferation and induced apoptosis. Our findings provide further evidence of the anti-tumor effects of WEPC and PA with potential pharmacological benefits for the treatment of cancer.
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