Induction of G2/M Arrest and Growth Inhibition by Aqueous Extract of Ajuga bracteosa (AEAB) in Human Nasopharyngeal and Pharyngeal Carcinoma Cell Lines

• Main Authors: Chih-Che Lin, 林志哲
• Other Authors: 徐怡強
• Format: Others
• Language: zh-TW
• Published: 2015
• Online Access: http://ndltd.ncl.edu.tw/handle/353vp9

碩士 === 長榮大學 === 醫學研究所 === 103 === Malignant tumor is leading mortality in Taiwan, therefore the prevention and therapy of cancer is more important now. In recent years, many studies have found that natural plant materials or biologically active ingredients has strong chemopreventive effects and may play an important role in chemotherapy . Ajuga bracteosa (Labiatae) is a common wild herb growing in open fields throughout Asia. The entire plant has been used to treat various diseases, including liver cirrhosis, sore throat, nasopharyngeal carcinoma, and arthritis. Traditionally, it was used to treat malaria in India. But the anti-tumor activities of Ajuga bracteosa were still unclear. In the study, we have investigated the anti-tumor effect of the aqueous extract of Ajuga bracteosa (AEAB) in human pharyngeal and nasopharyngeal carcinoma cell lines (Hone-1 and Detroit 562 cells) . Our results show that cell viability of Hone-1 and Detroit562 were decreased by treated with AEAB for 24 to 72 hours by the MTT assay. It’s in a dose-dependent and time course manner. We also used the flow cytometry with Propidium Iodide and Annexin V plus Propidium Iodide double staining assay to see whether the growth inhibition or apoptosis/necrosis induced by AEAB. The Hone-1 and Detroit562 cells treated with AEAB will go to cell cycle G2/M phase arrest but not apoptosis/necrosis. The mitotic index (MPM-2) detected by flow cytometry was elevated in both cell lines after treatment with AEAB. The elevated mitotic index meant that the cell cycle G2/M-related proteins were influenced by AEAB. We used Western blot and Q-PCR to detect the cell cycle G2/M-related proteins” Cyclin B1 and Cdk1” and its mRNA expressions. The results showed that the AEAB could induce Cyclin B1 and Cdk1 proteins and mRNA decrease in both cell lines. In this study, Ajuga bracteosa may be an effective treatment of nasopharyngeal carcinoma and pharyngeal carcinoma (or adjuvant chemotherapy), and simultaneously we discussed the tumor suppressor role of the human nasopharyngeal carcinoma and pharyngeal carcinoma to provide a new direction of cancer therapy and prevention.