The anti-hyperuricemic effect of the leaf essential oil of Cinnamomum osmophloeum Kanehiraand its active ingredients linalool and cinnamaldehyde in mice

• Main Authors: Hsing-Wen Tsai, 蔡幸紋
• Other Authors: Cheng-Tzu Liu
• Format: Others
• Language: zh-TW
• Published: 2015
• Online Access: http://ndltd.ncl.edu.tw/handle/73678142349563756605

碩士 === 中山醫學大學 === 營養學系碩士班 === 103 === It has been shown that the leave essential oil of Cinnamomum osmophloeum Kanehira (TC) and cinnamaldehyde, an bioactive composition of TC, reduced serum uric acid concentration in oxonate-induced hyperuricemia in mice. The aim of present study is to investigate the effect of TC and two of its compositions, linalool and cinnamaldehyde, on hyperuricemia-associated hyperglycemic and inflammatory condition in chronic and in acute hyperuricemia in mice. In study part I, male C57BL/6 mice were induced to be chronic hyperuricemia by the injection of uric acid (250 mg/kg BW/day, i.p.) for 4 consecutive weeks followed by receiving 7 consecutive days by gavage TC (13 mg/kg BW), Lin (2.6, 5.2 mg/kg BW), Cin (0.4, 0.9 mg/kg BW) or the vehicle (corn oil, 4 ml/kg BW). Normal control mice injected with an equal volume of vehicle received 7 consecutive days by gavage corn oil. Mice were then sacrificed by CO2 at 24 h after the final intervention. In study part II, male C57BL/6 mice received every other day for eight times by gavage Lin (2.6、5.2、10.4 mg / kg BW), Cin (0.4, 0.9, 1.8 mg/kg BW) or the vehicle (corn oil, 4 ml / kg BW) followed by the injection of potassium oxonate (375 mg / kg BW, OX, i.p.) to induce acute hyperuricemia. Normal control mice that received corn oil were injected with vehicle (10 ml/kg BW). Mice were then sacrificed by CO2 at one hour after the induction. The results of study part I showed that the intervention of all tested doses of Lin, Cin, and TC reduced the serum uric acid concentration and improved the metabolic condition, fasting blood glucose level, and HOMA-IR value in mice. In addition, Lin, Cin, and TC also reduced levels of nitrate/nitrite and IL-1β in kidney, pancreas and heart of these animals. As a result, renal function reflected by GFR was normalized by the intervention with Lin, Cin, and TC which is associated with normalized levels of the expression of NLRP3, ASC, caspase-1, TLR4, MyD88, and MD2 in hyperuricemia mice. The results of study part II showed that OX-induced elevation of blood uric acid level was prevented by Lin and Cin. Similarly, these two compounds reversed acute hyperuricemia-associated elevation of fasting blood glucose level and HOMA-IR value. In addition, Lin and Cin also prevented OX-induced elevation of levels of nitrate/nitrite and IL-1β in kidney, pancreas and heart in mice. In summary, Lin, Cin and TC showed prophylactic and therapeutic effect on acute- and chronic hyperuricemia-associated hyperglycemic and inflammatory condition, respectively, in mice.