Estrogen Receptor α mRNA important than E2 Is an Independent biomarker for Postmenopausal Osteoporosis

• Main Authors: Chi-Wen Chou, 周啟文
• Other Authors: Chung-Hung Tsai
• Format: Others
• Language: zh-TW
• Published: 2015
• Online Access: http://ndltd.ncl.edu.tw/handle/60012496909641838564

博士 === 中山醫學大學 === 醫學研究所 === 103 === Objective：Up- and down-regulation of the osteoclastogenesis response depends on the Estrogen/estrogen receptor (ER) signaling pathway. Previous reports have shown that promoter hypermethylation and gene polymorphism of ER α are risks for menopausal osteoporosis. No previous study has evaluated expression levels of ERα mRNA of menopausal osteoporosis using human subjects. We hypothesized that ERα mRNA expression may show less resistance to postmenopausal osteoporosis. Methods and Materials：In this study, we enrolled 86 women older than 45 years without menstruation and classified them as the menopausal group and classified another 86 women aged 25 to 45 years with regular menstruation as the childbearing age group. ERα mRNA levels in peripheral blood lymphocytes (PBLs) were analyzed by the quantitative real-time reverse-transcription polymerase chain reaction (QRT-PCR), and estrogen in serum was detected by ELISA. Results：We found that ERα mRNA levels in PBLs had a negative correlation with age and a positive correlation with hip and lumbar T-scores in the menopausal group but not in the childbearing age group. Additionally, multivariate analysis showed that older age (≥50 years), a low estradiol concentration (<45.6 pg/mL), and low ERα mRNA levels in PBLs (<200 copies/μg DNA) were associated with an approximately 46.80-, 5.41-, and 50-fold risk of osteoporosis. Conclusion：We conclude that ERα mRNA levels in PBLs could be used as an independent risk factor for menopausal osteoporosis and that they may be more important than age and serum estrogen levels.