| Summary: | 博士 === 中山醫學大學 === 醫學研究所 === 103 === N-Acetylcysteine (Nac) is an antioxidant administered in both oral and injectable forms in clinics. Here we used Nac topically on treating burn wounds in vitro and in vivo to investigate the mechanisms. In vitro, we detected glutathione levels, cell proliferation, migration, scratch-wound healing activities and the epithelialization-related proteins; matrix metalloproteinase-1 (MMP-1) and proteins involved in the formation pathway of MMP-1 of CCD-966SK cells treated with Nac. Various Nac concentrations (0.1, 0.5, and 1.0 mM) increased glutathione levels, cell viability, scratch-wound healing activities and migration abilities of CCD-966SK cells in a dose-dependent manner. MMP-1 expression of CCD-966SK cells treated with 1.0 mM Nac for 24 hrs was significantly increased. Levels of PI3K, PKC, Jak1, Stat3, c-Fos and Jun, but not Erk1/2, also significantly increased with dose dependence compared to the control. In addition, Nac induced collagenous expressions of MMP-1 via PKC/Stat3 signaling pathway. In vivo, the burn wound healing model of rats was applied to assess the stimulating activity and the histopathologic effects of Nac, with 3.0% Nac-treated wounds being found to show better characteristics on re-epithelialization. Our results demonstrated Nac potentially promoted wound healing activity. Nac may be a promising drug for accelerating burn wound healing.
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