| Summary: | 碩士 === 大葉大學 === 生物產業科技學系 === 102 === C. militaris is a precious medicinal fungi, that belongs to Clavicipitaceae, with ingredients similar to Cordyceps sinensis. C. militaris have multiple activities, such as antioxidation, anti-tumor, anti-inflummation, immunity adjustment effects and a variety of chemical constituents, including cordycepin, ergosterol and adenosine in these activities. Owing to the productive of natural C. sinensis is limited by the factors of environment and the climate, C. militaris have cultured a good succedaneum in the future. In this study, the nutritional requirements and culture conditions for the culture of three stains of C. militaris were investigated in an attempt to obtain optimal production of mycelia, polysaccharides, adenosine and cordycepin by three species.
The best growth in agar media was obtained at 25°C (60 mm colony diameter in 14 days). The initial agar media pH range from 6 to 8 was found to be the most favourable for mycelial growth. The most favourable carbon and nitrogen sources were lactose and peptone, respectively. Additionally, The results demonstrated that brown rice is better than polished rice in growth of fruit bodies in the solid state fermentation. However, the optimal cultural medium benefits the production enhancement of fruit bodies biomass and physiologically active components (cordycepin and adenosine) of C. militaris.
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