In vitro cultivation of curdlan-grafted poly(vinyl alcohol) with luffa aegyptiaca fiber for tissue engineering

• Main Authors: Chia-Chi Hsu, 許家齊
• Other Authors: Wen-Chuan Hsieh
• Format: Others
• Language: zh-TW
• Published: 2015
• Online Access: http://ndltd.ncl.edu.tw/handle/36876832013167150358

碩士 === 義守大學 === 生物科技學系 === 103 === Curdlan and luffa aegyptiaca fibers were grafted to poly(vinyl alcohol) (PVA) to form a porous scaffold. The grafted PVA-Curdlan 3D scaffold was then observed by Fourier transform infrared spectroscopy (FTIR). Grafting increased the water absorbency of the scaffold by 280%. Scanning electron microscopic (SEM) observations of the material revealed that the 3D scaffold was highly porous when formed using a homogenizer at 100 rpm. Compression testing revealed that, increasing the amount of Curdlan and adding luffa aegyptiaca fiber increased the strength of the 3Dscaffold to 15-35 × 10-3 MPa. Over 28 days, the 3D scaffold was degraded by various enzymes with a weight loss of up to 20-40%. In vitro tests revealed favorable cell proliferation and growth in a 3D scaffold.