| Summary: | 碩士 === 國立中興大學 === 化學系所 === 103 === Nanomaterials are very popular in biochemical sensor researches recently. In this study, gold nanoparticle (Au NP) decorated TiO2 nanowire (TiO2 NW) electrode was constructed to immobilize cytochrome c for H2O2 electrochemical biosensors. TiO2 NW has some particularities like its chemical inertness, rigidity, thermal stability, and good biocompatibility；Au NPs also have been attracting attentions for their good biocompatibility, high stability, and easy production. Au NPs act as good electron-transfer site and can easily pierce through the shells of proteins, thus the electron transfer pathway between the active centers inside cytochrome c and the electrode is shortened, promoting the electron-transfer rate. Cyt. c was immobilized on TiO2 by electrostatically interaction which can prevent proteins denaturating due to strong chemical bonding. And the electron-transfer path through one-dimensional structure TiO2 NW can reduce electrical signal loss due to disorderly path .
The surface coverage of cyt. c absorbed on TiO2 NW electrode is 40.5%，and the value is raising to 60.4% when using Au NP/TiO2 NW electrode. In addition, Au NPs also reduce the resistance of electrode and help cyt. c quickly response with H2O2 within 5s. The linear range of H2O2 detection was 0.1mM to 1.2mM . From these experiment results, Au NP/ TiO2 NW electrode will be a promising electrode material for third-generation biosensors.
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