Microbial Community Shifts and Treatment Efficiency of a Swine Wastewater feeding Lab-scale Mesophilic Anaerobic Fermentation Reactor

• Main Authors: Po-Chun Liu, 劉柏均
• Other Authors: Chun-Hsiung Hung
• Format: Others
• Language: zh-TW
• Published: 2015
• Online Access: http://ndltd.ncl.edu.tw/handle/92902133797905766488

碩士 === 國立中興大學 === 環境工程學系所 === 103 === This study is aimed to research the efficiency and microbial communities shift of anaerobic fermentor in 3-Step pig manure waste water treatment process, provided as a reference to improve anaerobic fermentor. The study is separated in 3 trial. Trail 1, use batch test to estimate the best temperature and total solid concentration. Trail 2, use the best parameter of trail 1 in a anaerobic continuous flow stirred tank reactor (CSTR) to test the best rotational speed. Trail 3, use the best parameter to modified manure wastewater treatment in the factory, monitor the the efficiency and microbial communities shift form start to steady state. In batch test, the 35℃ has the best performance, the cumulative gas production is 900 ml, concentration of methane is 60%. In vail 2, rotational speed between 30~70 rpm has the best performance, Efficiency of gas production is 1000 mL/Day, high rotational speed and no mixing has adverse effects to system. In vail 3, we use the best parameter to modified manure wastewater treatment in the factory, the efficiency of gas production improved significantly, the efficiency of gas production is approximately 2000 mL/Day at steady state. COD loading is relevant to the efficiency of gas production, the linear regression equation is y=0.0077x – 1.4838, R2=0.8295, 1 g COD can produced about 0.315 L biogass, it’s closel to the theoretical value 0.35 L. The system has complex microbial communities, the system has found many kinds of Clostridiales, Firmicutes and Enterobacteriaceae ( such as Firmicutes bacterium, Sphaerochaeta globose), play key roles in the hydrolysis and acidogenesis stages of degrading the complex organic matters in swine manure. Also found many species of Methanogens, such as Methanocorpusculum parvum, Methanoculleus chikugoensis, ethanolobus psychrophilus. They have different kinds of metabolism that can degrade a variety of organic compounds, the major metabolism in system is methanogenesis from H2/CO2. This study provides information on the efficiencies and microbial communities shift in anaerobic fermentor of 3-Step pig manure waste water treatment process.