Airway microbiome dynamic in chronic obstructive pulmonary disease patients with acute exacerbation and respiratory failure- A longitudinal study

• Main Authors: Sin-Yin Liu, 劉思吟
• Other Authors: Chieh-Chen Huang
• Format: Others
• Language: zh-TW
• Published: 2015
• Online Access: http://ndltd.ncl.edu.tw/handle/36293914599942083277

碩士 === 國立中興大學 === 生命科學系所 === 103 === Objectives: This study aimed to examine the pulmonary complex bacterial diversity dynamics in patients with chronic obstructive pulmonary disease (COPD) acute exacerbation and to explore the relationships between complex bacterial diversity dynamics and clinical outcomes in patients with COPD acute exacerbation. Materials & Methods: The serial endotracheal aspirates (EA) were collected via endotracheal tubes or tracheostomy to minimize upper airway contamination. We also collected anthropometric and demographic data, results of chest X-ray and pulmonary function tests, and COPD-associated clinical findings of the enrolled subjects. Using culture-independent molecular methods, including DNA extraction, PCR-16SrDNA, and new-generation sequence, the pulmonary complex bacterial diversity dynamics were analyzed. The relationships between complex bacterial diversity dynamics and clinical outcomes were also examined. Results: A total of 9 COPD patients with acute exacerbation-related respiratory failure were enrolled. Increased pulmonary complex bacterial diversity of serial EA samples, as determined by Simpson scores and Shannon scores, was found in 6subjects with weaning success. Decreased pulmonary complex bacterial diversity was found in 3 subjects with weaning failure. Conclusion: The dynamics of pulmonary complex bacterial diversity might predict the weaning outcomes in COPD patients with acute exacerbation-related respiratory failure.