Chlorella sorokiniana Health Supplement Enhances Recovery from Taxol-Induced Leukopenia

• Main Authors: Ya-Ching Yu, 游雅清
• Other Authors: Cheng-Chieh Lin
• Format: Others
• Language: zh-TW
• Published: 2015
• Online Access: http://ndltd.ncl.edu.tw/handle/05556424008780414336

碩士 === 國立中興大學 === 生命科學院碩士在職專班 === 103 === Chlorella sorokiniana (CS) is a single-cell green algae, green algae rich in nutrients, such as protein, amino acids, antioxidant enzymes and minerals. In addition,Chlorella sorokiniana can also cause a variety of beneficial pharmacological effects.Therefore this study is focused on evaluating the ability Chlorella sorokiniana extract in improving chemotherapy supressed bone marrow function. One day after Taxol treatment, laboratory mice had been given hot water extracted Chlorella every day within following 7 days(Based on subject weight, group A given 17mg/kg, group B given 34mg/kg), laboratory mice had been given hot water extracted Chlorella sorokiniana every day within three weeks (Based on its weight,. 50mg/kg). Compare with mice which received only Taxol treatment, white blood cell counts in peripheral blood which sampled 7 (Group A) and 3 (Group B) days after additional Chlorella extract treatment don''t show significant difference. On the other hand, neutrophil level for both groups had been significantly increased. In vitro assays showed that Chlorella sorokiniana enhanced the colony-forming ability of both granulocyte macrophage colony forming unit (GM-CFU) and osteogenic cells from bone marrow preparations and promoted the differentiation of bone marrow mesenchymal stromal cells into adipocytes, alkaline phosphatase–positive osteoblasts, and bone tissue.his result could be attributed to enhanced expression of Cbfa1 (core binding factor a) and BMP-2 (bone morphogenetic protein) with concurrent suppression of ODF (osteoclast differentiation factor/RANK [receptor activator of NF-κB]) ligand. In summary, Based on results of this experinment, Chlorella extract not only affects bone marrow and osteoblast differentiation but also enhances recovery of mice from Taxol treatment induced leukopenia.