| Summary: | 碩士 === 國立中興大學 === 生命科學系所 === 103 === Biofuel production is using renewable biomass as substrate to produce fuels such as hydrogen,ethanol and butanol. Lignocellulose is the most abundant renewable feedstock to produce biofuel. Lignocellulose is mainly composed of cellulose, hemicelluloses and lignin. However, glucose-based is surrounded by hemicellulose, lignin and pectin, which make it hard to be decomposed. This study was aimed to establish a functional bacterial consortia as a lignocellulose digestion system for biofuel production through serial repeated batch culture. The major bacterial composition of batch culture were termite gut isolated Bacillus pumilus MGB 5 and Clostridium acetobutylicum ATCC 824. This system does not require sterilization, aeration and shake.On an eight day incubation period the system could produce 275 ml /L H2, ethanol 1250 mg/L, butanol 71 mg/L, acetate acid 2275 mg/L, butyrate acid 661 mg/L and left 64% cellulose and hemicellulose from mango powder biomass. The major bacteria in these system are Enterobacteriaceae, Enterococcus, Clostridium, Enterobacter, Bacillus. In the experiment , we found out if we use 3% mango powder as carbon source which can produce higher butanol than using 1% glucose as carbon source.This simple system is very potential to replace the traditional system which use glucose as carbon source to produce hydrogen and butanol.
|
|---|
