Preventive Effect of Fat Accumulation by Curcuma phaeocaulis & Curcuma aromatica rhizomes in 3T3-L1 Preadipocytes and High Fat diet-induced Obesity C57BL/6J Mice
碩士 === 國立中興大學 === 食品暨應用生物科技學系所 === 103 === Obesity is caused by extra energy intake, which increase the size of adipocytes and make body unhealthy. The aim of this study was to investigate the preventive effects of fat accumulation by Curcuma phaeocaulis and Curcuma aromatica in vitro and in vivo. T...
Main Authors: | , |
---|---|
Other Authors: | |
Format: | Others |
Language: | zh-TW |
Published: |
2015
|
Online Access: | http://ndltd.ncl.edu.tw/handle/49533332298380193348 |
id |
ndltd-TW-103NCHU5253053 |
---|---|
record_format |
oai_dc |
spelling |
ndltd-TW-103NCHU52530532016-02-21T04:33:27Z http://ndltd.ncl.edu.tw/handle/49533332298380193348 Preventive Effect of Fat Accumulation by Curcuma phaeocaulis & Curcuma aromatica rhizomes in 3T3-L1 Preadipocytes and High Fat diet-induced Obesity C57BL/6J Mice 蓬莪蒁及春鬱金對3T3-L1前脂肪細胞及高脂飲食誘導肥胖之C57BL/6J小鼠不易形成體脂肪之探討 Ci-Sian Wang 王啟憲 碩士 國立中興大學 食品暨應用生物科技學系所 103 Obesity is caused by extra energy intake, which increase the size of adipocytes and make body unhealthy. The aim of this study was to investigate the preventive effects of fat accumulation by Curcuma phaeocaulis and Curcuma aromatica in vitro and in vivo. The in vitro results indicated that 50-100 g/ml CPO and 500g/ml CAE can significantly inhibit the lipid accumulation by oil O staining. RT-PCR (real-time polymerase chain reaction) analysis was used to estimate the gene expression of adipocytes differentiation and lipid metabolism. The results showed that 100 g/ml CPO treatment can decrease the expression of adipogenesis-related gene ACC (53.4±12.4%) and increase the expression of lipolysis-related gene CPT-1 (91.0±52.2%). 500g/ml CAE treatment can decrease the expressions of preadipocytes differentiation-related gene PPAR(54.5±14.5%) and ACC (67.4±8.8%), but increase the expression of CPT-1 (168.6±57.7%). The preventive effect of fat accumulation by Curcuma phaeocaulis and Curcuma aromatica powder (CPP and CAP) was evaluated in vivo. Eight-week-old male C57BL/6J mice were fed normal diet as control group (n=10) and high-fat diet to induce obesity (n=70) as obese group for ten weeks. Subsequently, obese group mice were randomly divided into seven groups (n=10), including high-fat diet group (HFD); low, medium and high-doses of Curcuma phaeocaulis group (LCP、MCP and HCP) and low, medium and high-doses of Curcuma aromatica group (LCA、MCA and HCA). These mice were fed for eight weeks and sacrificed, the serum biochemical parameters and histopathological analysis were assayed. The results showed that administration of CA and CP powders reduced the ratio of body weights range from 19.4-28.8 % and 14.4-36.5 % (p<0.05), and body fat ratio from 1.6-3.1 % and 0.4-4.0 %. Moreover, the size of adipocytes was smaller in all treatment groups than HFD group. The serum parameters of AST, ALT and leptin levels in treatment groups were reduced compared to HFD group. Histopathological analysis revealed that LCP, MCP, HCP, LCA, MCA and HCA decreased the pathological change of fatty infiltration in liver from score 4.5±0.8 (HFD group) to score 2.6±1.6, 3.1±1.2, 2.1±1.3, 3.8±1.0, 3.4±1.4 and 1.3±1.3, respectively. In conclusion, essential oil of Curcuma phaeocaulis and ethanol extract of Curcuma aromatica can inhibit lipid accumulation in 3T3-L1 preadipocytes by decreasing the expression of ACC and increasing the expression of CPT gene. Furthermore, oral administration of CP and CA powder can reduce the body weight, body fat ratio, fat accumulation in epididymal fat and severity of fatty liver in the mice. Shen-Shih Chiang 蔣慎思 2015 學位論文 ; thesis 109 zh-TW |
collection |
NDLTD |
language |
zh-TW |
format |
Others
|
sources |
NDLTD |
description |
碩士 === 國立中興大學 === 食品暨應用生物科技學系所 === 103 === Obesity is caused by extra energy intake, which increase the size of adipocytes and make body unhealthy. The aim of this study was to investigate the preventive effects of fat accumulation by Curcuma phaeocaulis and Curcuma aromatica in vitro and in vivo. The in vitro results indicated that 50-100 g/ml CPO and 500g/ml CAE can significantly inhibit the lipid accumulation by oil O staining. RT-PCR (real-time polymerase chain reaction) analysis was used to estimate the gene expression of adipocytes differentiation and lipid metabolism. The results showed that 100 g/ml CPO treatment can decrease the expression of adipogenesis-related gene ACC (53.4±12.4%) and increase the expression of lipolysis-related gene CPT-1 (91.0±52.2%). 500g/ml CAE treatment can decrease the expressions of preadipocytes differentiation-related gene PPAR(54.5±14.5%) and ACC (67.4±8.8%), but increase the expression of CPT-1 (168.6±57.7%). The preventive effect of fat accumulation by Curcuma phaeocaulis and Curcuma aromatica powder (CPP and CAP) was evaluated in vivo. Eight-week-old male C57BL/6J mice were fed normal diet as control group (n=10) and high-fat diet to induce obesity (n=70) as obese group for ten weeks. Subsequently, obese group mice were randomly divided into seven groups (n=10), including high-fat diet group (HFD); low, medium and high-doses of Curcuma phaeocaulis group (LCP、MCP and HCP) and low, medium and high-doses of Curcuma aromatica group (LCA、MCA and HCA). These mice were fed for eight weeks and sacrificed, the serum biochemical parameters and histopathological analysis were assayed. The results showed that administration of CA and CP powders reduced the ratio of body weights range from 19.4-28.8 % and 14.4-36.5 % (p<0.05), and body fat ratio from 1.6-3.1 % and 0.4-4.0 %. Moreover, the size of adipocytes was smaller in all treatment groups than HFD group. The serum parameters of AST, ALT and leptin levels in treatment groups were reduced compared to HFD group. Histopathological analysis revealed that LCP, MCP, HCP, LCA, MCA and HCA decreased the pathological change of fatty infiltration in liver from score 4.5±0.8 (HFD group) to score 2.6±1.6, 3.1±1.2, 2.1±1.3, 3.8±1.0, 3.4±1.4 and 1.3±1.3, respectively. In conclusion, essential oil of Curcuma phaeocaulis and ethanol extract of Curcuma aromatica can inhibit lipid accumulation in 3T3-L1 preadipocytes by decreasing the expression of ACC and increasing the expression of CPT gene. Furthermore, oral administration of CP and CA powder can reduce the body weight, body fat ratio, fat accumulation in epididymal fat and severity of fatty liver in the mice.
|
author2 |
Shen-Shih Chiang |
author_facet |
Shen-Shih Chiang Ci-Sian Wang 王啟憲 |
author |
Ci-Sian Wang 王啟憲 |
spellingShingle |
Ci-Sian Wang 王啟憲 Preventive Effect of Fat Accumulation by Curcuma phaeocaulis & Curcuma aromatica rhizomes in 3T3-L1 Preadipocytes and High Fat diet-induced Obesity C57BL/6J Mice |
author_sort |
Ci-Sian Wang |
title |
Preventive Effect of Fat Accumulation by Curcuma phaeocaulis & Curcuma aromatica rhizomes in 3T3-L1 Preadipocytes and High Fat diet-induced Obesity C57BL/6J Mice |
title_short |
Preventive Effect of Fat Accumulation by Curcuma phaeocaulis & Curcuma aromatica rhizomes in 3T3-L1 Preadipocytes and High Fat diet-induced Obesity C57BL/6J Mice |
title_full |
Preventive Effect of Fat Accumulation by Curcuma phaeocaulis & Curcuma aromatica rhizomes in 3T3-L1 Preadipocytes and High Fat diet-induced Obesity C57BL/6J Mice |
title_fullStr |
Preventive Effect of Fat Accumulation by Curcuma phaeocaulis & Curcuma aromatica rhizomes in 3T3-L1 Preadipocytes and High Fat diet-induced Obesity C57BL/6J Mice |
title_full_unstemmed |
Preventive Effect of Fat Accumulation by Curcuma phaeocaulis & Curcuma aromatica rhizomes in 3T3-L1 Preadipocytes and High Fat diet-induced Obesity C57BL/6J Mice |
title_sort |
preventive effect of fat accumulation by curcuma phaeocaulis & curcuma aromatica rhizomes in 3t3-l1 preadipocytes and high fat diet-induced obesity c57bl/6j mice |
publishDate |
2015 |
url |
http://ndltd.ncl.edu.tw/handle/49533332298380193348 |
work_keys_str_mv |
AT cisianwang preventiveeffectoffataccumulationbycurcumaphaeocauliscurcumaaromaticarhizomesin3t3l1preadipocytesandhighfatdietinducedobesityc57bl6jmice AT wángqǐxiàn preventiveeffectoffataccumulationbycurcumaphaeocauliscurcumaaromaticarhizomesin3t3l1preadipocytesandhighfatdietinducedobesityc57bl6jmice AT cisianwang péngéshùjíchūnyùjīnduì3t3l1qiánzhīfángxìbāojígāozhīyǐnshíyòudǎoféipàngzhīc57bl6jxiǎoshǔbùyìxíngchéngtǐzhīfángzhītàntǎo AT wángqǐxiàn péngéshùjíchūnyùjīnduì3t3l1qiánzhīfángxìbāojígāozhīyǐnshíyòudǎoféipàngzhīc57bl6jxiǎoshǔbùyìxíngchéngtǐzhīfángzhītàntǎo |
_version_ |
1718195491288645632 |