| Summary: | 碩士 === 國立中興大學 === 園藝學系所 === 103 === Tolumnia are indigenous to the Caribbean islands, and have extensive habitats in that region. Plants are miniature oncidium with triangular succulent leaves that form small fan shaped growths and lack of a pseudobulb. The hybrids present a surprising array of colors and have been hybridized for a relative short term to reach flowering size which enabled breeders to breed important advances in a few years. The aims of this experiment were developed an efficient tissue culture system for clonal propagation of tolumnias orchid and tested the effects of the plant growth regulators 6-benzyladenine (BA) and flower stalk node positions on mass propagation of Tolumnia Snow Fairy and Tolumnia GS248. After 16 weeks of culture, the 4th node position of flower stalks in Tolumnia Snow Fairy showed the highest (57.1%) shoot formation rate, which the 3rd node position is differentiation in Tolumnia GS248. While, both of them showed higher response on ½ MS salt basal medium supplemented with 4 mg/L BA. Not only flower stalk nodes but also the effects of flower stalks stage on the efficiency of PLBs induction from Tolumnia Snow Fairy’s flower buds explants were examined. After 8 weeks of culture, the highest response rate of PLBs showed of 70% of the lateral flower buds in second stage when they were cultured on ½ MS salt basal medium with 4 mg/L BA. Next, plantlets establishment and transplantations derived from flower stalk node were cultured and transplanted to greenhouse condition presented 100% and 54.2% of the plantlets of Tolumnia Snow Fairy and Tolumnia GS248 survived after 90 days of transfer. Furthermore, only 3 progenies out of 4 crosses numbers were chosen that including, 3 plants of RS cross, 2 plants of 101-186 and 1 plant of 101-233 cross. This opens up the route for in vitro culture methods that does not damage the mother plants and also proved useful for future studies elucidating for floral tissues involved in subsequent differentiation processes of shoot formation and PLBs induction. Finally, the progenies were selected and hope to use in flower stems or flower buds culture for multiplying of progenies.
|
|---|
