| Summary: | 碩士 === 國立中興大學 === 微生物暨公共衛生學研究所 === 103 === Orf virus (ORFV), belonging to parapoxvirus, causes skin lesions in infected sheep and goats. Due to the restricted host range, the skin tropism, the absence of systemic virus spread, and the short-lived ORFV vector-specific immunity allowing repeated immunizations, it has been successfully used as a novel viral vector system for expressing foreign antigens to prevent infectious diseases of swine, rabbit, and avian. Hence, study herein aimed to establish ORFV as a bivalent vaccine platform for goat. In 2008, goatpox (GP) outbreaks caused substantial loss in the production and productivity of goats in Taiwan. Considering that control of goatpox virus (GPV) infection relies on foreign vaccine, the goal of current study is to construct a recombinant ORFV that would express goatpox virus P32, the major immunogenic protein. To do so, coding regions of GPV P32 and eGFP that serves as a selection marker were inserted between the flanking sequences, i.e. open reading frame 127 and 128 of the ORFV genome. By homologous recombination, recombinant ORFV with GPV p32 gene (GPV P32-ORFV) was generated. At the present, the presence and expression of GPV P32 gene were confirmed by polymerase chain reaction and western blot analysis, respectively. The immune characteristics of GPV P32-ORFV will be further tested in animal models.
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