| Summary: | 博士 === 國立成功大學 === 化學工程學系 === 103 === In recent years, the need of rapid bio-detection and analysis in many territories such as medical research, pathogen detection, biomass production, environmental monitoring, homeland security have been increasing drastically. The microfluidic devices constructed by exploiting the MEMS (Micro-Electro-Mechanical System) technology can well meet the demands because they possess several advantages such as small amount sample required, faster analysis, high throughput, multiplexing, relatively simple procedure for sample pretreatment and increased portability. In this study, we addressed and developed some of microfluidic techniques for the applications in the abovementioned areas. To effectively select and identify the algal strains with high-lipid content and potentially monitor the cultivation process in biofuel production, we constructed the batch and continuous dielectrophoresis (DEP) microchip to better understand the flow behavior of microalgae with different lipid contents under the non-uniform AC electric field and utilize this information to perform continuous sorting and detection of microalgae. For fast analysis of pathogens, the microfluidic chips with microneedle array were fabricated and performed under the non-uniform AC electric field. By combining both the effects of dielectrophoresis and surface enhanced Raman spectroscopy (SERS), the Staphylococcus aureus (S. aureus) was first effectively separated from the red blood cells (RBCs) and concentrated at the tips of the microneedle array, followed by analysis through SERS. For better heterogeneous SERS detection, one of the important parameters is the amount of the “hot spots” on the SERS substrates. To realize the effect of “hot spots” on SERS detection, several black silicon (BS) surfaces consisting of different tip densities which correspond to different amount of “hot spots” were constructed via inductively coupled plasma etching and gold evaporation. It was concluded that the SERS spectra of rhodamine 6G molecules at solution concentration as low as 10 fM can be obtained by using the optimized BS surface. Finally, a relative rapid, simple and reliable technique to fabricate microneedles by 3D polydimethylsiloxane (PDMS) etching was proposed and developed, which can be used to construct the microfluidic chip with microneedle array and in the applications of transdermal drug delivery.
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