The role of disulfideisomerase domain containing protein in the drug tolerance mechanism of Acanthamoeba

• Main Authors: Tao-ShenLiu, 劉道生
• Other Authors: Wei-Chen Lin
• Format: Others
• Language: zh-TW
• Published: 2015
• Online Access: http://ndltd.ncl.edu.tw/handle/98270798521515529331

碩士 === 國立成功大學 === 微生物及免疫學研究所 === 103 === In this study, we found that disulfideisomerase domain containing protein (PDI) ACA1_189420 is up-regulated by PHMB treatment in Acanthamoeba castellanii NCKH_D, a highly PHMB-tolerant strain. Furthermore, PDI affects the expression level of Ribonucleotide reductase (RNR) and DNA replication in Acanthamoeba as the role of Thioredoxin (Trx) in mammalian cell. NCKH_D was vulnerable to PHMB after PDI-specific siRNA silencing, which proved the relationship between PDI and drug tolerance. These results show that PDI is an important gene for drug tolerance ability in PHMB-tolerant Acanthamoeba. In addition, we also revealed the PDI signaling transduction in Acanthamoeba.