The role of angiotensin II type 1 receptor (AT1R) in ultrafine particle-induced lung injury
碩士 === 國立成功大學 === 環境醫學研究所 === 103 === In this study, we investigated the role of angiotensin II type 1 receptor (AT1R) in ultrafine particle induced lung injury. Male ICR mice were oropharyngeal aspirated with ufCB (0, 100, 200 μg/mouse). For inhibitory study, a group of mice were injected with AT1R...
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ndltd-TW-103NCKU55200042016-08-22T04:17:51Z http://ndltd.ncl.edu.tw/handle/99628908097145450902 The role of angiotensin II type 1 receptor (AT1R) in ultrafine particle-induced lung injury 血管緊張素 II 第一型受體於超細微粒下誘導肺部損傷中所扮演的角色 Wan-TingLin 林婉婷 碩士 國立成功大學 環境醫學研究所 103 In this study, we investigated the role of angiotensin II type 1 receptor (AT1R) in ultrafine particle induced lung injury. Male ICR mice were oropharyngeal aspirated with ufCB (0, 100, 200 μg/mouse). For inhibitory study, a group of mice were injected with AT1R antagonist (ZD7155; 20 mg/kg) intraperitoneally 30 min before ufCB aspirated. The total cell counts, differential counts and total protein were measured in bronchoalveolar (BAL) fluid. Enzyme-linked immunosorbent assay (ELISA) was used to measure vascular endothelial growth factor (VEGF) and tumor necrosis factor alpha (TNF-α) in BAL fluid, and VEGF in blood. The results show that exposure to ufCB caused a dose-dependent increased the total cell counts, the numbers of neutrophils and total protein in BAL fluid, with the maximal increase at 21 hr. The data indicate that ufCB caused pulmonary inflammatory response and alveolar-capillary permeability. TNF-α was significantly increased at 6 hr. Furthermore, ufCB induced significant productions of VEGF in BAL fluid over the study period, with a maximal increase at 16 hr. Pretreatment with ZD7155 significantly reduced the total cell counts and neutrophils in BAL fluid. ZD7155 also reduced the expression of TNF-α, total protein and VEGF. The results from our study demonstrate that ufCB exposure induced pulmonary inflammatory response and alveolar-capillary permeability may be mediated by AT1R. Chih-Ching Chang 張志欽 2015 學位論文 ; thesis 49 zh-TW |
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碩士 === 國立成功大學 === 環境醫學研究所 === 103 === In this study, we investigated the role of angiotensin II type 1 receptor (AT1R) in ultrafine particle induced lung injury. Male ICR mice were oropharyngeal aspirated with ufCB (0, 100, 200 μg/mouse). For inhibitory study, a group of mice were injected with AT1R antagonist (ZD7155; 20 mg/kg) intraperitoneally 30 min before ufCB aspirated. The total cell counts, differential counts and total protein were measured in bronchoalveolar (BAL) fluid. Enzyme-linked immunosorbent assay (ELISA) was used to measure vascular endothelial growth factor (VEGF) and tumor necrosis factor alpha (TNF-α) in BAL fluid, and VEGF in blood. The results show that exposure to ufCB caused a dose-dependent increased the total cell counts, the numbers of neutrophils and total protein in BAL fluid, with the maximal increase at 21 hr. The data indicate that ufCB caused pulmonary inflammatory response and alveolar-capillary permeability. TNF-α was significantly increased at 6 hr. Furthermore, ufCB induced significant productions of VEGF in BAL fluid over the study period, with a maximal increase at 16 hr. Pretreatment with ZD7155 significantly reduced the total cell counts and neutrophils in BAL fluid. ZD7155 also reduced the expression of TNF-α, total protein and VEGF. The results from our study demonstrate that ufCB exposure induced pulmonary inflammatory response and alveolar-capillary permeability may be mediated by AT1R.
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author2 |
Chih-Ching Chang |
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Chih-Ching Chang Wan-TingLin 林婉婷 |
author |
Wan-TingLin 林婉婷 |
spellingShingle |
Wan-TingLin 林婉婷 The role of angiotensin II type 1 receptor (AT1R) in ultrafine particle-induced lung injury |
author_sort |
Wan-TingLin |
title |
The role of angiotensin II type 1 receptor (AT1R) in ultrafine particle-induced lung injury |
title_short |
The role of angiotensin II type 1 receptor (AT1R) in ultrafine particle-induced lung injury |
title_full |
The role of angiotensin II type 1 receptor (AT1R) in ultrafine particle-induced lung injury |
title_fullStr |
The role of angiotensin II type 1 receptor (AT1R) in ultrafine particle-induced lung injury |
title_full_unstemmed |
The role of angiotensin II type 1 receptor (AT1R) in ultrafine particle-induced lung injury |
title_sort |
role of angiotensin ii type 1 receptor (at1r) in ultrafine particle-induced lung injury |
publishDate |
2015 |
url |
http://ndltd.ncl.edu.tw/handle/99628908097145450902 |
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