The study of CaHGT6 , CaORF19.7566 , CaCDR4 and CaCDR3CDR4 double mutant null mutations on the morphogenesis and growth condition of Candida albicans

• Main Authors: Lee, Ko-Wei, 李克威
• Other Authors: Yang, Yun-Liang
• Format: Others
• Language: zh-TW
• Published: 2014
• Online Access: http://ndltd.ncl.edu.tw/handle/21643907846848017669

碩士 === 國立交通大學 === 生物科技系所 === 103 === Candida albicans is an opportunistic fungal pathogen, usually as a commensal in humans. However, when the host becomes immunocompromised, it may cause superficial and systemic infections. Candida albicans can switch from a yeast form into filamentous forms. It is known that the morphological transition is regulated by the transcription factors CaEfg1p and/or CaCph1p. The cph1/cph1 efg1/efg1 double mutant is defective in filamentous growth, locked in the yeast form, and became avirulent in a mouse model. This indicates that virulence is related to the morphogenesis in Candida albicans. The expressions of CaHGT6, CaORF19.7566 and CaCDR4 were affected by transcriptional factors CaEfg1p and/or CaCph1p in Candida albicans based on the result of expression profile comparison. These three genes may be related to hyphae growth or virulence, and they are putative transporter genes. In this study, I used SAT 1 flipper cassette to construct homozygous knock-out strains of these three genes and determined the effect on morphogenesis and their growth rates. In conclusion, there is no significant difference between the homozygous strain and the wild-type strain in their morphogenesis ability under the tested condition. And there is no difference between the homozygous strain and the wild-type strain in their growth rates under the tested condition.