| Summary: | 碩士 === 國立彰化師範大學 === 生物技術研究所 === 103 === Termitomyces is a symbiotic fungus of the termite Odontotermes formosanus. The fungus-growing termites maintain their symbionts on specially constructed fungus combs, and the termite workers consume the nitrogen-rich fungal nodules that grow on the combs. Laccases are essential to the saprophytic lifestyle of wod-degraing fungi. Laccase is a copper-containing phenoloxidase. Because of its broad substrate specificity, laccase can be utilized in many industrial processes. In this study, we collected mycelia from fruiting bodies of the basidiomycete Termitomyces sp. TerY. Several putative laccase inducers were tested for their ability to stimulate laccase production in liquid culture of Termitomyces mycelia and the major laccase released into the culture supernatant was purified. The result showed that the maximum laccase activity was obtained after 25 days of incubation. The formation of extracellular laccase could be considerably stimulated by 1 mM of CuSO4. Laccase was purified by ultrafiltration, anion-exchange chromatography and immobilized metal ion affinity chromatography. Kinetic parameters of laccase toward 2, 2‟-azinobis 3-ethylbenzathiozone-6-sulfonic acid (ABTS) were Km = 0.0185 mM， kcat = 170 sec-1.
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