Development of a Bivalent Subunit Vaccine against Porcine Reproductive and Respiratory Syndrome Virus and Porcine Circovirus Type 2 infection

• Main Authors: Yen-Jie Wang, 王妍絜
• Other Authors: Wen-Hsing Chang
• Format: Others
• Language: zh-TW
• Online Access: http://ndltd.ncl.edu.tw/handle/38393232860017317860

碩士 === 國立嘉義大學 === 生物農業科技學系研究所 === 103 === Postweaning multisystemic wasting syndrome (PMWS) is endemic in many swine producing countries and continues to be a major cause of wasting disease in swine worldwide. Porcine circovirus type 2’s (PCV2) sole infection is shown to be sufficient for the occurrence of PMWS. And it is markedly increase in mortality due to co-infection with porcine reproductive and respiratory syndrome virus (PRRSV). This research appllies PCV2 and PRRSV genome sequences to develop an expression vector containing the two viruses’ genes that can be expressed in the E. coli expression system. After immunizing mice with the purified fusion protein, individual mouse serum is subject to ELISA and dot blotting assay. The recombinant PCV2-PRRSV fusion protein is proven to be capable of inducing mice to generate the specific antibodies against PCV2 and PRRSV respectively. These data have laid the foundation of developing a bivalent subunit vaccine for preventing the PMWS occurrences in the future.