Effects of organic additives and Thidiazuron on rhizome proliferation and differentiation of Cymbidium goeringii var. goeringii

• Main Authors: Ming-De Sung, 宋明德
• Other Authors: Cherng-Kang Liao
• Language: zh-TW
• Online Access: http://ndltd.ncl.edu.tw/handle/63855063843219496970

碩士 === 國立嘉義大學 === 農藝學系研究所 === 103 === The purpose of this study was to establish an micropropagation protocol of Cymbidium goeringii var. goeringii. Rhizomes were cultured on modified MS medium (mMS) medium with 3 g L-1 banana, potato and coconut powder respectively (BPC) for 8 weeks for proliferation. 3.1 rhizomes per explant was proliferated. 1.0 cm rhizome apices were cut and transferred to mMS medium with 0.25 mg L-1 Thidiazuron (1-phenyl- 3-(1,2,3-thiadiazol-5-yl)urea, TDZ) for 4 weeks for bud formation, and 2.7 buds per explant were formed. For optimizing the plantlets, only apical buds were cut and transferred to mMS medium with 3 g L-1 Hyponex® No. 1 (N:P:K = 6:7:19) for 4 weeks. 50% of apical buds developed into plantlets and 42% developed into shoots. This indicated that the proliferation and differentiation of rhizome were promoted by BPC culture. 0.5 cm rhizome apices, 1.0 cm and 2.0 cm rhizome section were culture in mMS liquid medium with 0.5 mg L-1 TDZ, and there were 2.8, 3.9, 5.8 buds to be formed respectively. To promote micropropagation efficiency, apical buds which were cultured with 0.25 mg L-1 TDZ were cultured on mMS medium with 3.0 g L-1 Hyponex® No. 1, and 93.8% developed into plantlets. When TDZ culture was increased from 4 weeks to 6 weeks, the percentage of plantlets were decreased 5%~40%. Those indicated that buds formation in 0.25 mg L-1 TDZ for 4 weeks were suitable. According to those protocols, 85% apical bud of hybrid C. goeringii var. goeringii “Chun-lan × Wu-feng” developed into plantlets, but in the case of “Wu-feng × Chun-lan”, it’s only 60%. This indicated that the cross combination play a role in apical bud development. Those shoots and plantlets developed from apical buds were more than 80% seedling when they were transferred to mMS solid medium with 50 g L-1 banana mud. Those results suggested that the micropopagation protocol established by this study is efficient for commercial production and breeding for Cymbidium goeringii var. goeringii.