| Summary: | 碩士 === 國立東華大學 === 海洋生物研究所 === 103 === Aquaria worldwide have been exhibiting millions of fish in the tanks. The demand for marine ornamental fish is getting higher, but artificial breeding technology is not mature. More than 95% of the fish are wild-caught. In the National Museum of Marine Biology and Aquarium, many coral reef fish can spawn naturally in the artificial environment. Therefore, we aim to collect these fish egg to breed them and put them back into the fish tank for self-sustainability. Previous study has shown that inorganic fertilization of nitrogen and phosphorus (N:700 µg/L, P:100 µg/L) can increase the survival of larval fish. In this study, we further increase the nitrogen to 1000 µg/L while maintaining the phosphorus at 100 µg/L to enhance phytoplankton growth in order to increase fish survival, and compare it to the previous method. Natural sea water was drawn from the coast and fertilized, and fish eggs were added in the 5th day. We measured nutrients, chlorophyll a, physico-chemical parameters, as well as zooplankton species, quantity and density. Stomach contents of the larvae were also examined. The results showed that the chlorophyll a and the zooplankton density was not significantly different between the two treatments (P>0.05). Larva fish survival rate in high N:P (N:1000 µg/L, P:100 µg/L) was 1.37% and the low N:P (N:700 µg/L, P:100 µg/L) was 2.58%. There was no significant difference between the two treatments (P>0.05).
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