Utilizing highly conserved sequences of dengue virus envelope protein to develop efficacious dengue virus vaccines
碩士 === 國防醫學院 === 生物化學研究所 === 103 === Dengue virus has four serotypes. Because one kind of infection by other serotypes if more serotypes viral infections, and it will produce severe symptoms. Therefore, the vaccine must be able to simultaneously neutralize the four serotypes of dengue virus to be...
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ndltd-TW-103NDMC01070102017-02-19T04:30:48Z http://ndltd.ncl.edu.tw/handle/02469364336245280852 Utilizing highly conserved sequences of dengue virus envelope protein to develop efficacious dengue virus vaccines 利用登革病毒膜蛋白質的高保留性序列發展有效登革疫苗之研究 Chun-wei Chen 陳俊瑋 碩士 國防醫學院 生物化學研究所 103 Dengue virus has four serotypes. Because one kind of infection by other serotypes if more serotypes viral infections, and it will produce severe symptoms. Therefore, the vaccine must be able to simultaneously neutralize the four serotypes of dengue virus to be effective against the virus. So up to now still no effective vaccine against the dengue virus. The reports found in the paper, there is a conserved sequence in dengue virus membrane protein domain II bc loop sequence(The 73 to 79 amino acids RCPTQGE). It can be induced against four serotypes of the dengue virus neutralizing antibodies. In this study, we inserted a conserved sequence to Japanese encephalitis virus (JEV) infectious clone to investigate conserved sequence of dengue virus protection force. We used mice administered the recombinant virus into mice inducing an immune response, and collected mouse sera to observe whether there has neutralizing antibodies. It was found that the limited antibody titers could identify conserved sequence. Therefore we changed the way, we increased the number of repetitions of the conserved sequences to insert JEV infectious clone and considered whether more effective to induce an immune response. Li-chen Yen 顏莉蓁 2015 學位論文 ; thesis 55 zh-TW |
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碩士 === 國防醫學院 === 生物化學研究所 === 103 === Dengue virus has four serotypes. Because one kind of infection by other serotypes if more serotypes viral infections, and it will produce severe symptoms. Therefore, the vaccine must be able to simultaneously neutralize the four serotypes of dengue virus to be effective against the virus. So up to now still no effective vaccine against the dengue virus. The reports found in the paper, there is a conserved sequence in dengue virus membrane protein domain II bc loop sequence(The 73 to 79 amino acids RCPTQGE). It can be induced against four serotypes of the dengue virus neutralizing antibodies. In this study, we inserted a conserved sequence to Japanese encephalitis virus (JEV) infectious clone to investigate conserved sequence of dengue virus protection force. We used mice administered the recombinant virus into mice inducing an immune response, and collected mouse sera to observe whether there has neutralizing antibodies. It was found that the limited antibody titers could identify conserved sequence. Therefore we changed the way, we increased the number of repetitions of the conserved sequences to insert JEV infectious clone and considered whether more effective to induce an immune response.
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author2 |
Li-chen Yen |
author_facet |
Li-chen Yen Chun-wei Chen 陳俊瑋 |
author |
Chun-wei Chen 陳俊瑋 |
spellingShingle |
Chun-wei Chen 陳俊瑋 Utilizing highly conserved sequences of dengue virus envelope protein to develop efficacious dengue virus vaccines |
author_sort |
Chun-wei Chen |
title |
Utilizing highly conserved sequences of dengue virus envelope protein to develop efficacious dengue virus vaccines |
title_short |
Utilizing highly conserved sequences of dengue virus envelope protein to develop efficacious dengue virus vaccines |
title_full |
Utilizing highly conserved sequences of dengue virus envelope protein to develop efficacious dengue virus vaccines |
title_fullStr |
Utilizing highly conserved sequences of dengue virus envelope protein to develop efficacious dengue virus vaccines |
title_full_unstemmed |
Utilizing highly conserved sequences of dengue virus envelope protein to develop efficacious dengue virus vaccines |
title_sort |
utilizing highly conserved sequences of dengue virus envelope protein to develop efficacious dengue virus vaccines |
publishDate |
2015 |
url |
http://ndltd.ncl.edu.tw/handle/02469364336245280852 |
work_keys_str_mv |
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