Utilizing highly conserved sequences of dengue virus envelope protein to develop efficacious dengue virus vaccines

• Main Authors: Chun-wei Chen, 陳俊瑋
• Other Authors: Li-chen Yen
• Format: Others
• Language: zh-TW
• Published: 2015
• Online Access: http://ndltd.ncl.edu.tw/handle/02469364336245280852

碩士 === 國防醫學院 === 生物化學研究所 === 103 === Dengue virus has four serotypes. Because one kind of infection by other serotypes if more serotypes viral infections, and it will produce severe symptoms. Therefore, the vaccine must be able to simultaneously neutralize the four serotypes of dengue virus to be effective against the virus. So up to now still no effective vaccine against the dengue virus. The reports found in the paper, there is a conserved sequence in dengue virus membrane protein domain II bc loop sequence(The 73 to 79 amino acids RCPTQGE). It can be induced against four serotypes of the dengue virus neutralizing antibodies. In this study, we inserted a conserved sequence to Japanese encephalitis virus (JEV) infectious clone to investigate conserved sequence of dengue virus protection force. We used mice administered the recombinant virus into mice inducing an immune response, and collected mouse sera to observe whether there has neutralizing antibodies. It was found that the limited antibody titers could identify conserved sequence. Therefore we changed the way, we increased the number of repetitions of the conserved sequences to insert JEV infectious clone and considered whether more effective to induce an immune response.