Small Interference RNA Targeting Transforming Growth Factor-beta Type I Receptor in Scar Prevention

• Main Authors: Wang, Yi-Wen, 王怡文
• Other Authors: Dai, Niann-Tzyy
• Format: Others
• Language: zh-TW
• Published: 2015
• Online Access: http://ndltd.ncl.edu.tw/handle/88030968598704054921

博士 === 國防醫學院 === 醫學科學研究所 === 103 === Hypertrophic scars tends to appear on skin tissue where recover from severe trauma or burn injury. It often affects the patient’s appearance and physical comfort. Hypertrophic scars is caused by excess deposition of extracellular matrix and hyper-proliferation of fibroblast which triggered by over expression of transforming growth factor beta (TGF-β) and its receptor, TGF-β type I receptor (TGFBRI). For the downregulation of TGFBRI gene expression, my work herein applied TGFBRI small interfering RNA (siRNA) on human dermal scar fibroblast and the rabbit hypertrophic scarring model. As the result, the downregulation of TGFBRI abundance in vitro might constrain the cell proliferation of fibroblast and decrease the expression of extracellular matrix. In the animal model, the result consistently revealed that TGFBRI siRNA decreased the scar elevation index and improved the appearance of the wound on rabbit-ear. Hence, my dissertation suggests that TGFBRI siRNA could decrease scar hypertrophy and is potentially feasible for clinical implementation.