| Summary: | 碩士 === 國立宜蘭大學 === 化學工程與材料工程學系碩士班 === 103 === In this study, montmorillonite (MMT) and chitosan (CS) composite films were prepared for surface modification of 316L stainless steels. Attenuated total reflectance fourier transform infrard rays spectrometer (ATR-FTIR), energy dispersive x-ray spectroscopy (EDS) and x-ray photoelectron spectrometer (XPS) results showed that. CS/MMT films were grafted on the surface of 316L stainless steel. Besides, there were abundant SiO2 distributed on the surface of the composite films without accumulation of MMT. Water contact angle tests revealed that the water contact angle of the composite films increased with the increasing MMT content of the composite films, indicating that the hydrophobicity characteristic of the films became more significantly at the same time. Scanning electron microscope images showed that the surface of CS films was smooth and dense. The surface became rougher with the increase of the amount of MMT in the composite films. However, some cracks appeared on the surface of the composite films with high MMT contents. X-ray photoelectron spectrometer versatile spectrum showed that there were significant Si 2s and Si 2p and Al 2s and Al 2p peaks obtained in the composite films. The amount of the N-C=O functional group decreased when the MMT content in films increased. The reducing amount of the N-C=O functional group decrease the opportunity of the reactions between primary amine amino acid and bovine serum albumin, leading to the lower surface protein adsorption of the composite films. However when the MMT amount exceeded 8 g, the amount of the protein adsorption on the composite films increased due to the cracks formed on the surface. Preliminary toxicity assessments showed that CS/MMT composite films did not exhibit significant toxicity to Escherichia coli. The corrosion current of the CS/MMT composite films were relatively lower that of the 316L stainless steel, suggesting that the deposited CS/MMT composite films can reduce the corrosion rate of the 316L stainless steel. According to our results, CS/MMT composite films did not present significant toxicity, exhibited good hydrophobic property, reduced the protein adsorption and corrosion rate on the surface of 316L stainless steel. Therefore, CS/MMT composite films were promising for biomedical applications.
Keywords: Chitosan; Montmorillonite; Biomaterials; Protein adsorption; X-ray photoelectron spectrometer; Water contact angle.
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