Developing a Novel Bio-stimulant for Treating Dyeing Wastewater

• Main Authors: Bo-Sian Chen, 陳柏憲
• Other Authors: Shyi-Tien Chen
• Format: Others
• Language: zh-TW
• Published: 2015
• Online Access: http://ndltd.ncl.edu.tw/handle/52474189585584649828

碩士 === 國立高雄第一科技大學 === 環境與安全衛生工程研究所 === 103 === Textile dyeing and finishing wastewater varies greatly due to the diversity of the dyeing processes used on different fabrics. Biological and/or chemical treatment methods are often used in treating this kind of wastewater. In our experiences chemical oxidation needs a lot of oxidant and catalysts is hardly economically effective. On the other hand, the biological treatment removes color and chemical oxygen demand (COD) is a rather feasible alternative to use. However, the toxicity of dyes and other auxiliaries and lack of bacterial nutrients retards the growth of microbes in the system, resulting in the poor performance of COD removal. Animal blood contains rather rich ingredients of nutrients and is readily available in a slaughter house. This study aimed to treat dyeing wastewater using a biological mean with three following objectives: (1) to study the effectiveness of industrial carbon sources and nutrients on COD removal, (2) to explore the potential use of pig blood as a bio-stimulant, and (3) to determine the feasible amount of the pretreated blood in treating dyeing wastewater. Batch or semi-continuous type reactors were employed to study the effectiveness of COD removal by various amount of the bio-stimulant added. The main techniques used to break the blood cells included oxidation, sonication and anaerobic fermentation. The results showed that glucose was readily used by the bacteria from a dyeing wastewater plant. The additions of nitrogen and phosphate, and metals helped in bacterial growth as well. It appeared that the system lack of nutrients that were not present in its wastewater. By adding EDTA-2Na at 40 mM in blood, no coagulation was observed in the first day of blood withdrawn from the slaughter house. Ten times of bacterial growth in batch tests were observed on day 3 if using fermentation over the oxidation and sonication to lysis blood. This indicates that fermentation was a superior method in blood lysis. Also addition of 0.72 mL or more of the fermented blood in a 400-mL batch reactor resulted in 1,000 mg/L of cell growth in three days. The use of fermented blood as a bio-stimulant in boosting cell growth seemed applicable.