Expression of Genes Associated with Innate Immunity in Peripheral Blood Mononuclear Cells from Pigs Co-infected with Porcine Reproductive and Respiratory Syndrome Virusand Porcine Circovirus Type 2

• Main Authors: Dong Van Hieu, 童文孝
• Other Authors: Wen-Bin Chung
• Format: Others
• Language: en_US
• Published: 2015
• Online Access: http://ndltd.ncl.edu.tw/handle/69267138735806736241

碩士 === 國立屏東科技大學 === 獸醫學系所 === 103 === Porcine reproductive and respiratory syndrome virus (PRRSV) and porcine circovirus type 2 (PCV2) are the two major pathogens, causing most severe economic losses in swine industry worldwide. Field and experimental studies have shown that co-infection of PRRSV and PCV2 in pigs were quite common and thus the interactions between both viruses in the host might be critical to the pathogenesis of the disease. The exact interaction mechanisms are unknown and remained to be elucidated. In this study, the expression of cytokine mRNA as well as Toll like receptor signaling molecules associated with innate immunity in peripheral blood mononuclear cells (PBMCs) from pigs infected with PRRSV and/or PCV2 were investigated. Cytokine mRNA and TLR signaling molecule expression were determined by quantitative real-time polymerase chain reaction. Co-infection of pigs with PRRSV and PCV2 led to up-regulated expression of IL-1β and IL-10 mRNA and down-regulated expression of TNF-α and IL-6 in PBMCs of pigs co-infected with PRRSV and PCV2. An increased expression of CD14 mRNA was noticed in pigs co-infected with PRRSV and PCV2. In contrast, there were down-regulated expression of A20, Bcl-3, FoxP3, IRAK-M, RANTES and SAMR1 and that might contribute to the increased expression of proinflammatory cytokines in pigs co-infected with PRRSV and PCV2. A down-regulated expression of IRF-1 and IRF-3 might also suppress the expression of IFNs that leads to the enhanced replication of PCV2 in pigs co-infected with PRRSV and PCV2.