Macrophage uptake of platelets-H. pylori is mainly IgG-mediated

• Main Authors: Hsiao-Li Fan, 樊曉勵
• Other Authors: Angela Chen
• Format: Others
• Language: zh-TW
• Published: 2015
• Online Access: http://ndltd.ncl.edu.tw/handle/rx9897

碩士 === 國立中山大學 === 生物醫學研究所 === 103 === One of the most important physiological functions of platelets (PLTs) is clotting in the wound. Recent studies have revealed that PLTs also play important roles in innate immunity. We’re interested in the association between H. pylori and PLTs due to patients with thrombocytopenia often accompanied with H. pylori infection. There is growing evidence showed that the PLTs count recovered after H. pylori eradication therapy. It’s known that adhesion of some strains of H. pylori to PLT leaded to PLT activation, aggregation and apoptosis. However, the role of PLT in the innate immunity of macrophage eliminating H. pylori, and in the association between phagocytosis and H. pylori-induced thrombocytopenia remains unclear. We therefore investigated the association between the H. pylori infected PLTs and the uptake of PLTs by macrophage in this studies. Firstly, it’s observed that the expression of phosphatidylserine (PS), a molecular marker for macrophage clearance, increased on washed PLTs in response to H. pylori and lipopolysaccharide (LPS) of H. pylori stimulation. Secondly, the uptake of H. pylori infected washed PLTs by macrophage was slightly enhances, but it was slightly decreased when LPS was used instead of H. pylori. Finally, we investigated the role of H. pylori antibody. Results showed that H. pylori positive sera significantly enhanced the phagocytosis of washed PLTs in response to some strains of H. pylori infection. Our results suggest the increased uptake of PLTs-H. pylori by macrophage was mainly IgG-mediated. It would lead to decrease in platelet counts. Our previous data on H. pylori-induced PLTs aggregation and apoptosis and the results of this study will provide further understandings on the pathogenesis of H. pylori-induced thrombocytopenia. It will further assist physicians in diagnosis and making therapeutic decisions on the H. pylori infected patients with unknown reason thrombocytopenia.